Temporal and spatial analysis of cartilage proteoglycan core protein gene expression during limb development by in situ hybridization.
Temporal and spatial analysis of cartilage proteoglycan core protein gene expression during limb development by in situ hybridization.
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通过原位杂交对肢体发育过程中软骨蛋白多糖核心蛋白基因表达进行时空分析。
DOI：
10.1016/0012-1606(88)90144-3
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发表时间：
1988
影响因子：
2.7
通讯作者：
Tanzer,ML
中科院分区：
文献类型：
--
作者：
Mallein-Gerin,F;Kosher,RA;Upholt,WB;Tanzer,ML
As limb mesenchymal cells differentiate into chondrocytes they initiate the synthesis of a cartilage-specific sulfated proteoglycan, cartilage-characteristic type II collagen, and other cartilage-specific proteins. In the present study,in situhybridization with a32P-labeled cloned cDNA probe complementary to mRNA encoding the core protein of cartilage proteoglycan has been used to visualize and localize the accumulation of cartilage proteoglycan core protein mRNA sequences during development of the chick limb budin vivo. When the probe was hybridized to sections through 7-day (stage 32) limbs, an intense hybridization signal was observed over the well-differentiated cartilage rudiments of the limb, while no signal above background was observed over nonchondrogenic tissues including muscle, loose connective tissue, and epidermis. At early stages of limb development, an accumulation of silver grains representing hybridizable core protein mRNA first became detectable in the proximal central core of the limb where the prechondrogenic condensation of mesenchymal cells that characterizes the onset of cartilage differentiation was occurring. In fact, the pattern of silver grain accumulation closely followed the pattern of mesenchymal cell condensation, and no hybridizable core protein mRNA sequences were detectable in the limb bud prior to condensation. Cartilage-characteristic type II collagen mRNA was colocalized with core protein mRNA in the condensing central core of the limb suggesting that the genes for these two major constituents of cartilage matrix are coordinately regulated at the onset of chondrogenesis. Furthermore, the appearance of hybridizable core protein mRNA was closely followed by the appearance of the protein for which it codes as detected by immunohistochemical staining with monospecific antibody. These observations support the hypothesis that at the initial stages of limb chondrogenesis core protein gene expression is controlled primarily at the transcriptional level.
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作者：
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DOI：
10.1016/0378-4347(88)80122-1
发表时间：
1988
期刊：
Journal of chromatography
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影响因子：
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作者：
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通讯作者：
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