Specific markers and properties of synovial mesenchymal stem cells in the surface, stromal, and perivascular regions.
Specific markers and properties of synovial mesenchymal stem cells in the surface, stromal, and perivascular regions.
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DOI：
10.1186/s13287-018-0870-9
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发表时间：
2018-05-02
影响因子：
7.5
通讯作者：
Sekiya I
中科院分区：
文献类型：
--
作者：
Mizuno M;Katano H;Mabuchi Y;Ogata Y;Ichinose S;Fujii S;Otabe K;Komori K;Ozeki N;Koga H;Tsuji K;Akazawa C;Muneta T;Sekiya I
滑膜间充质干细胞（MSC）是软骨和半月板再生的一种有吸引力的细胞来源。滑膜组织在组织学上可分为三个区域：表面、基质和血管周围区域，但滑膜间充质干细胞的定位尚未得到充分研究。我们确定了每个区域的特异性标记，并比较了滑膜中每个区域来源的MSC的特性。用19种抗体检测了6名骨关节炎患者滑膜表面、基质和血管周围区域的免疫染色强度。鉴定特异性标志物，并分选来自每个区域的滑膜细胞。比较了来自三个区域的滑膜间充质干细胞的增殖、表面标志物表达、软骨形成、钙化和脂肪形成潜力。我们选择表面区域的滑膜细胞为CD 55 + CD 271 −，基质区域为CD 55 − CD 271 −，血管周围区域为CD 55 − CD 271+。分选的细胞与非造血谱系细胞的比率在表面区域中为5%，在基质区域中为70%，在血管周围区域中为15%。滑膜细胞在血管周围部分有最大的增殖潜力。扩增后，表面标志物的表达谱和脂肪形成潜力是相似的，但软骨和钙化的潜力是更高的滑膜间充质干细胞来源于血管周围区域比那些来自表面和基质区域。我们确定了从滑膜表面、基质和血管周围区域分离滑膜细胞的特异性标记物。在三个区域中，血管周围区域的滑膜间充质干细胞具有最高的增殖和软骨形成潜力。
Synovial mesenchymal stem cells (MSCs) are an attractive cell source for cartilage and meniscus regeneration. Synovial tissue can be histologically classified into three regions; surface, stromal and perivascular region, but the localization of synovial MSCs has not been fully investigated. We identified markers specific for each region, and compared properties of MSCs derived from each region in the synovium. The intensity of immunostaining with 19 antibodies was examined for surface, stromal, and perivascular regions of human synovium from six osteoarthritis patients. Specific markers were identified and synovial cells derived from each region were sorted. Proliferation, surface marker expression, chondrogenesis, calcification and adipogenesis potentials were compared in synovial MSCs derived from the three regions. We selected CD55+ CD271− for synovial cells in the surface region, CD55− CD271− in the stromal region, and CD55− CD271+ in the perivascular region. The ratio of the sorted cells to non-hematopoietic lineage cells was 5% in the surface region, 70% in the stromal region and 15% in the perivascular region. Synovial cells in the perivascular fraction had the greatest proliferation potential. After expansion, surface marker expression profiles and adipogenesis potentials were similar but chondrogenic and calcification potentials were higher in synovial MSCs derived from the perivascular region than in those derived from the surface and stromal regions. We identified specific markers to isolate synovial cells from the surface, stromal, and perivascular regions of the synovium. Synovial MSCs in the perivascular region had the highest proliferative and chondrogenic potentials among the three regions.
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DOI：
10.2174/1874312901105010100
发表时间：
2011
期刊：
The open rheumatology journal
影响因子：
--
作者：
Smith MD
通讯作者：
Smith MD
影响因子：
3.7
作者：
Nakagawa Y;Muneta T;Otabe K;Ozeki N;Mizuno M;Udo M;Saito R;Yanagisawa K;Ichinose S;Koga H;Tsuji K;Sekiya I
通讯作者：
Sekiya I
影响因子：
4.2
作者：
Matsukura Y;Muneta T;Tsuji K;Koga H;Sekiya I
通讯作者：
Sekiya I
影响因子：
5.9
作者：
Mabuchi, Yo;Morikawa, Satoru;Harada, Seiko;Niibe, Kunimichi;Suzuki, Sadafumi;Renault-Mihara, Francois;Houlihan, Diarmaid D.;Akazawa, Chihiro;Okano, Hideyuki;Matsuzaki, Yumi
通讯作者：
Matsuzaki, Yumi
影响因子：
4.2
作者：
Sekiya, Ichiro;Muneta, Takeshi;Horie, Masafumi;Koga, Hideyuki
通讯作者：
Koga, Hideyuki