Mesenchymal stem cells in synovial fluid increase after meniscus injury.
Mesenchymal stem cells in synovial fluid increase after meniscus injury.
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DOI：
10.1007/s11999-013-3418-4
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发表时间：
2014-05
影响因子：
4.2
通讯作者：
Sekiya I
中科院分区：
文献类型：
--
作者：
Matsukura Y;Muneta T;Tsuji K;Koga H;Sekiya I
尽管相对罕见，但即使在无血管区域也观察到半月板损伤的自发愈合。这可能与关节滑液中存在间充质干细胞有关。本研究旨在探讨半月板损伤后膝关节滑液中是否存在间充质干细胞。滑膜液取自22例半月板损伤患者术前膝关节和8例无膝关节损伤史的志愿者。将滑液的细胞部分培养14天，然后分析多谱系潜能和间充质干细胞特征性表面抗原的呈递。比较两组细胞的集落形成率和增殖能力。在损伤膝关节的滑液中观察到具有间充质干细胞特征的细胞，其程度比未损伤膝关节大得多。从半月板损伤的膝关节滑液中获得的集落形成细胞具有多向分化潜能，其表面抗原决定簇与间充质干细胞相同。膝关节损伤后1mL滑液中的平均集落形成数为250个，高于健康志愿者的0.5个（p < 0.001）。每单位滑液中的菌落数与伤后时间呈正相关（r = 0.77，p < 0.001）。半月板损伤后膝关节滑液中存在间充质干细胞。间充质干细胞在半月板损伤的滑液中的数量高于正常膝关节。每单位滑液体积的菌落总数与伤后时间呈正相关。我们目前的人体研究和之前的动物研究表明，半月板损伤后滑液中的间充质干细胞增加可能有助于半月板自发愈合。
Although relatively uncommon, spontaneous healing from a meniscus injury has been observed even within the avascular area. This may be the result of the existence of mesenchymal stem cells in synovial fluid. The purpose of this study was to investigate whether mesenchymal stem cells existed in the synovial fluid of the knee after meniscus injury. Synovial fluid was obtained from the knees of 22 patients with meniscus injury just before meniscus surgery and from 8 volunteers who had no history of knee injury. The cellular fraction of the synovial fluid was cultured for 14 days followed by analysis for multilineage potential and presentation of surface antigens characteristic of mesenchymal stem cells. Colony-forming efficiency and proliferation potential were also compared between the two groups. Cells with characteristics of mesenchymal stem cells were observed in the synovial fluid of injured knees to a much greater degree than in uninjured knees. The colony-forming cells derived from the synovial fluid of the knee with meniscus injury had multipotentiality and surface epitopes identical to mesenchymal stem cells. The average number of colony formation, obtained from 1 mL of synovial fluid, in meniscus-injured knees was 250, higher than that from healthy volunteers, which was 0.5 (p < 0.001). Total colony number per synovial fluid volume was positively correlated with the postinjury period (r = 0.77, p < 0.001). Mesenchymal stem cells were found to exist in synovial fluid from knees after meniscus injury. Mesenchymal stem cells were present in higher numbers in synovial fluid with meniscus injury than in normal knees. Total colony number per synovial fluid volume was positively correlated with the postinjury period. Our current human study and previous animal studies suggest the possibility that mesenchymal stem cells in synovial fluid increase after meniscus injury contributing to spontaneous meniscus healing.
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影响因子：
4
作者：
Shirasawa, S;Sekiya, I;Muneta, T
通讯作者：
Muneta, T
影响因子：
--
作者：
Jones, Elena A.;Crawford, Aileen;McGonagle, Dennis
通讯作者：
McGonagle, Dennis
DOI：
10.1302/0301-620x.92b1.22629
发表时间：
2010-01-01
影响因子：
--
作者：
Dutton, A. Q.;Choong, P. F.;Hui, J. H. P.
通讯作者：
Hui, J. H. P.
影响因子：
--
作者：
Englund, Martin;Guermazi, Ali;Roemer, Frank W.;Aliabadi, Piran;Yang, Mei;Lewis, Cora E.;Torner, James;Nevitt, Michael C.;Sack, Burton;Felson, David T.
通讯作者：
Felson, David T.
影响因子：
5.3
作者：
Kim, Sunny;Bosque, Jose;Marder, Richard
通讯作者：
Marder, Richard