Identification and characterization of the RouenBd1987 Babesia divergens Rhopty-Associated Protein 1.
Identification and characterization of the RouenBd1987 Babesia divergens Rhopty-Associated Protein 1.
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DOI：
10.1371/journal.pone.0107727
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lobo CA
中科院分区：
文献类型：
--
作者：
Rodriguez M;Alhassan A;Ord RL;Cursino-Santos JR;Singh M;Gray J;Lobo CA
人类巴贝斯虫病是由几种巴贝斯虫属的一种引起的，这些巴贝斯虫属的一种是由硬蜱传播的，硬蜱根据有能力的动物宿主的存在而具有不同的地理分布。巴贝斯虫病的病理学，如疟疾，是寄生虫血症的结果，通过巴贝斯虫寄生虫在患者的红细胞中的周期性复制而发展，尽管症状通常是非特异性的。我们已经从B的人类分离株中鉴定出编码棒状体相关蛋白-1（RAP-1）的基因。divergens，Rouen 1987，并在分子和细胞水平上表征了其蛋白产物。与其他巴氏杆菌RAP-1同源物一致，BdRAP-1在寄生虫棒状体中表达为46 kDa蛋白，表明在红细胞侵袭中可能起作用。天然BdRAP-1与未鉴定的红细胞受体结合，该红细胞受体在性质上似乎是非唾液酸化和非蛋白质的，但我们没有发现体外抗rRAP 1抗体的生长显著减少，这突出了B的可能性。趋异因子能够使用替代途径进行入侵，或者BdRAP-1在入侵过程中具有替代、互补的作用。由于寄生虫识别并随后侵入宿主细胞的能力是临床疾病的核心，因此表征和理解参与这些步骤的巴贝虫衍生蛋白的作用对于开发有效的预防具有极大的意义。
Human babesiosis is caused by one of several babesial species transmitted by ixodid ticks that have distinct geographical distributions based on the presence of competent animal hosts. The pathology of babesiosis, like malaria, is a consequence of the parasitaemia which develops through the cyclical replication of Babesia parasites in a patient's red blood cells, though symptoms typically are nonspecific. We have identified the gene encoding Rhoptry-Associated Protein −1 (RAP-1) from a human isolate of B. divergens, Rouen1987 and characterized its protein product at the molecular and cellular level. Consistent with other Babesia RAP-1 homologues, BdRAP-1 is expressed as a 46 kDa protein in the parasite rhoptries, suggesting a possible role in red cell invasion. Native BdRAP-1 binds to an unidentified red cell receptor(s) that appears to be non-sialylated and non-proteinacious in nature, but we do not find significant reduction in growth with anti-rRAP1 antibodies in vitro, highlighting the possibility the B. divergens is able to use alternative pathways for invasion, or there is an alternative, complementary, role for BdRAP-1 during the invasion process. As it is the parasite's ability to recognize and then invade host cells which is central to clinical disease, characterising and understanding the role of Babesia-derived proteins involved in these steps are of great interest for the development of an effective prophylaxis.
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