Urine chemokines indicate pathogenic association of obesity with BPH/LUTS.
Urine chemokines indicate pathogenic association of obesity with BPH/LUTS.
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DOI：
10.1007/s11255-015-0992-2
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发表时间：
2015-07
影响因子：
2
通讯作者：
Fowke, Jay H.
中科院分区：
文献类型：
--
作者：
Tyagi, Pradeep;Motley, Saundra S.;Kashyap, Mahendra;Pore, Subrata;Gingrich, Jeffrey;Wang, Zhou;Yoshimura, Naoki;Fowke, Jay H.
与良性前列腺增生（BPH）一致的下尿路症状（LUTS）的高患病率与肥胖和前列腺炎症相关。在这里，我们研究了是否可以在BPH/LUTS患者的正常排尿中测量与肥胖和前列腺炎症相关的趋化因子，以证明肥胖和BPH/LUTS之间的机械关联。将入组纳什维尔男性健康研究的BPH/LUTS患者的冷冻尿液标本送往匹兹堡大学进行盲法分析。30例患者的AUA-SI（>7或≤7）和前列腺增大（<40，40-60，>60 cc）被阻断。临床参数，包括年龄，前列腺大小，药物来自图表审查。使用Luminex™ xMAP®技术和用于NGF的ELISA测量解冻尿液中的CXC趋化因子（CXCL-1、CXCL-8和CXCL-10）、CC趋化因子（CCL 2和CCL 3）和sIL-1 ra。尿中的CCL 2水平是其他六种蛋白质的数倍，其中CCL 3在不到四分之一的患者中可检测到。BPH患者尿sIL-1 ra和CXCL-8水平与BMI和腰围的增加显著相关。CXCL-8显示与总体AUA-SI评分以及阻塞性（p = 0.08）症状分项评分的边缘相关性。前列腺体积与尿CXCL-10呈负相关，且呈轻微相关（p = 0.09）。尿中CXCL-8、CXCL-10和sIL-1 ra水平分别与LUTS严重程度、前列腺大小和肥胖有不同程度的相关性。尿液中的这些发现与过去对前列腺分泌物中趋化因子水平的研究一致，并证明了无创测量尿液中趋化因子对BPH/LUTS患者进行客观分类的潜力。
High prevalence of lower urinary tract symptoms (LUTS) consistent with benign prostate hyperplasia (BPH) is associated with obesity and prostatic inflammation. Here, we investigated whether chemokines associated with obesity and prostatic inflammation can be measured in normally voided urine of BPH/LUTS patients to demonstrate the mechanistic association between obesity and BPH/LUTS. Frozen urine specimens of BPH/LUTS patients enrolled in the Nashville Men's Health Study were sent for blinded analysis to University of Pittsburgh. Thirty patients were blocked by their AUA-SI (>7 or ≤7) and prostatic enlargement (<40, 40–60, >60 cc). Clinical parameters including age, prostate size, and medications were derived from chart review. CXC chemokines (CXCL-1, CXCL-8, and CXCL-10), CC chemokines (CCL2 and CCL3), and sIL-1ra were measured in thawed urine using Luminex™ xMAP® technology and ELISA for NGF. Urinary CCL2 levels were several fold higher compared with the other six proteins, of which CCL3 was detectable in less than one-fourth of patients. Urine levels of sIL-1ra and CXCL-8 were significantly associated with increasing BMI and waist circumference in BPH patients. CXCL-8 showed a marginal association with overall AUA-SI scores, as well as obstructive (p = 0.08) symptom sub-scores. Prostate volume was inversely and marginally associated with urinary CXCL-10 (p = 0.09). Urine levels of CXCL-8, CXCL-10, and sIL-1ra were associated with varying degrees with LUTS severity, prostate size, and obesity, respectively. These findings in urine are consistent with past studies of chemokine levels from expressed prostatic secretions and demonstrate the potential of noninvasively measured chemokine in urine to objectively classify BPH/LUTS patients.
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通讯作者：
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