Low-potential iron-sulfur centers in photosystem I: an X-ray absorption spectroscopy study.
Low-potential iron-sulfur centers in photosystem I: an X-ray absorption spectroscopy study.
复制标题
光系统 I 中的低电势铁硫中心：X 射线吸收光谱研究。
DOI：
10.1021/bi00411a018
复制
发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
M. Klein
中科院分区：
文献类型：
--
作者：
A. McDermott;V. Yachandra;R. Guiles;R. Britt;S. Dexheimer;K. Sauer;M. Klein
我们测量了菠菜和一种嗜热蓝细菌聚球藻（Synechococcus sp.）的光系统I（PS I）制剂中Fe的X射线吸收光谱，表征PS I中作为电子受体的Fe配合物的结构。这些受体包括中心A和B，它们可能是典型的[4Fe-4S]铁氧化还原蛋白，以及X。X的结构尚不清楚，但其电子顺磁共振（EPR）谱表明它是[2Fe-2S]或[4Fe-4S]铁氧还蛋白或铁醌类物质。铁的X射线吸收K边和扩展X射线吸收精细结构（EXAFS）谱表明，反应中心中的11-14个Fe原子基本上都以Fe-S中心的形式存在，12个Fe原子中不超过1个是八面体或氧配位的Fe。这表明，除了A和B之外，还存在另外的Fe-S簇，其可能是X。我们的EXAFS光谱不能充分模拟的[4Fe-4S]铁氧化还原蛋白与典型的键长和无序参数的混合物，因为铁背散射的振幅是小的，然而，出色的模拟数据是一致的混合物的[2Fe-2S]铁氧化还原蛋白和[4Fe-4S]铁氧化还原蛋白，或异常扭曲的[4Fe-4S]集群。我们假定[2Fe-2S]或畸变[4Fe-4S]心是X。聚球藻和菠菜的PS I制剂的X射线吸收光谱基本上是不可区分的。
We have measured the X-ray absorption spectra of Fe in photosystem I (PS I) preparations from spinach and a thermophilic cyanobacterium, Synechococcus sp., to characterize structures of the Fe complexes that function as electron acceptors in PS I. These acceptors include centers A and B, which are probably typical [4Fe-4S] ferredoxins, and X. The structure of X is not known, but its electron paramagnetic resonance (EPR) spectrum has generated the suggestions that it is either a [2Fe-2S] or [4Fe-4S] ferredoxin or an Fe-quinone species. The iron X-ray absorption K-edge and iron extended X-ray absorption fine structure (EXAFS) spectra reveal that essentially all of the 11-14 Fe atoms present in the reaction center are present in the form of Fe-S centers and that not more than 1 atom out of 12 could be octahedral or oxygen-coordinated Fe. This suggests that, besides A and B, additional Fe-S clusters are present which are likely to be X. Our EXAFS spectra cannot be simulated adequately by a mixture of [4Fe-4S] ferredoxins with typical bond lengths and disorder parameters because the amplitude of Fe backscattering is small; however, excellent simulations of the data are consistent with a mixture of [2Fe-2S] ferredoxins and [4Fe-4S] ferredoxins, or with unusually distorted [4Fe-4S] clusters. We presume that the [2Fe-2S] or distorted [4Fe-4S] centers are X. The X-ray absorption spectra of PS I preparations from Synechococcus and spinach are essentially indistinguishable.
细胞色素氧化酶的结构特点及反应机制：铁和铜X射线吸收精细结构。
影响因子：
3.4
作者：
Powers,L;Chance,B;Ching,Y;Angiolillo,P
通讯作者：
Angiolillo,P
球形红假单胞菌反应中心 Fe2 的电子结构。
影响因子：
3.4
作者：
Eisenberger,P;Okamura,MY;Feher,G
通讯作者：
Feher,G
DOI：
10.1016/s0021-9258(18)47500-x
发表时间：
1987-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Beckmann;P. Ljungdahl;J. L. Lopez;B. Trumpower
通讯作者：
J. Beckmann;P. Ljungdahl;J. L. Lopez;B. Trumpower