Effects of condensates from volcanic fumaroles and cigarette smoke extracts on airway epithelial cells.
Effects of condensates from volcanic fumaroles and cigarette smoke extracts on airway epithelial cells.
复制标题
DOI：
10.1007/s13577-023-00927-1
复制
发表时间：
2023-09
期刊：
影响因子：
4.3
通讯作者：
Pace, Elisabetta
中科院分区：
文献类型：
--
作者：
Di Sano, Caterina;Di Vincenzo, Serena;Lo Piparo, Doriana;D'Anna, Claudia;Taverna, Simona;Lazzara, Valentina;Pinto, Paola;Sortino, Francesco;Pace, Elisabetta
火山气载产物对气道上皮内环境稳定的影响在很大程度上是未知的。本研究评估了火山富马洛尔浓缩物（FC）单独或与香烟烟雾提取物（CSE）联合对气道上皮细胞（16 HBE和A549）的影响。采用气相色谱法和高效液相色谱法分析了FC的化学组成。将细胞暴露于FC并评估IL-33和IL-8。通过评估细胞代谢/细胞活力、线粒体应激、细胞凋亡/细胞坏死和细胞增殖，评价FC和CSE对细胞损伤的影响。FC含有：水蒸气（70-97%），CO2（3-30%），酸性气体（H2S，SO2，HCl，HF）约1%。FC增加了细胞内IL-33，但在测试的细胞系中不同地调节IL-33和IL-8基因表达和IL-8释放。FC（无/有CSE）：（a）增加16 HBE中的细胞代谢/细胞活力，而降低A549中的细胞代谢/细胞活力;（B）增加两种细胞类型中的线粒体应激。与单独CSE相比，FC与CSE增加了A549中的细胞坏死。CSE抑制16 HB、E细胞增殖，而增加A549细胞增殖，FC可抵消这两种细胞的作用。总体而言，在气道上皮细胞中存在CSE的情况下，FC诱导与代谢重编程相关的促炎特征，而无相关毒性。在线版本包含补充材料，可通过10.1007/s13577-023-00927-1获得。
The impact of volcanic airborne products on airway epithelium homeostasis is largely unknown. This study assessed the effects of volcanic Fumarole Condensates (FC) alone or combined with Cigarette Smoke Extracts (CSE) on airway epithelial cells (16HBE and A549). Chemical composition of FC was analyzed by gas chromatography and HPLC. Cells were exposed to FC and IL-33 and IL-8 were assessed. The effects of FC and CSE on cell injury were evaluated assessing cell metabolism/cell viability, mitochondrial stress, cell apoptosis/cell necrosis, and cell proliferation. FC contained: water vapor (70–97%), CO2 (3–30%), acid gases (H2S, SO2, HCl, HF) around 1%. FC increased the intracellular IL-33 but differently modulated IL-33 and IL-8 gene expression and IL-8 release in the tested cell lines. FC without/with CSE: (a) increased cell metabolism/cell viability in 16HBE, while decreased it in A549; (b) increased mitochondrial stress in both cell types. FC with CSE increased cell necrosis in A549 in comparison to CSE alone. CSE reduced cell proliferation in 16HB,E while increased it in A549 and FC counteracted these effects in both cell types. Overall, FC induce a pro-inflammatory profile associated to a metabolic reprogramming without a relevant toxicity also in presence of CSE in airway epithelial cells. The online version contains supplementary material available at 10.1007/s13577-023-00927-1.
登录
查看更多内容
DOI：
10.1042/bj20081386
发表时间：
2009-01-01
期刊：
The Biochemical journal
影响因子：
--
作者：
Murphy MP
通讯作者：
Murphy MP
DOI：
10.1016/s1387-2656(05)11004-7
发表时间：
2005-01-01
期刊：
BIOTECHNOLOGY ANNUAL REVIEW, VOL 11
影响因子：
--
作者：
Berridge, MV;Herst, PM;Tan, AS
通讯作者：
Tan, AS
DOI：
10.4049/jimmunol.1003020
发表时间：
2011-04-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kouzaki H;Iijima K;Kobayashi T;O'Grady SM;Kita H
通讯作者：
Kita H
影响因子：
4.5
作者：
Di Vincenzo, Serena;Sangiorgi, Claudia;Pace, Elisabetta
通讯作者：
Pace, Elisabetta
影响因子：
64.8
作者：
BAUBRON, JC;ALLARD, P;TOUTAIN, JP
通讯作者：
TOUTAIN, JP