The effects of tetracycline concentrations on tetracycline resistance genes and their bacterial hosts in the gut passages of earthworms (Eisenia fetida) feeding on domestic sludge
The effects of tetracycline concentrations on tetracycline resistance genes and their bacterial hosts in the gut passages of earthworms (Eisenia fetida) feeding on domestic sludge
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四环素浓度对以生活污泥为食的蚯蚓（Eisenia fetida）肠道内四环素抗性基因及其细菌宿主的影响
DOI：
10.1007/s11356-019-06495-y
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发表时间：
2019-10
影响因子：
5.8
通讯作者：
Kang Yijun
中科院分区：
文献类型：
--
作者：
Wang Yue;Yin Zhifeng;Zhao Haitao;Hu Jian;Kang Yijun
蚯蚓堆肥被认为是减少污泥中四环素耐药基因(TRGS)的一种可行方法。然而，不同肠道途径的蚯蚓可能会影响TRGS的命运，以及这一过程是否受到四环素(TC)浓度的影响，还需要进一步研究。在这项研究中，我们研究了TC浓度对蚯蚓肠道TRGS和细菌群落变化的影响，并通过定量PCR和Illumina高通量测序确定了这些细菌群落。在TC浓度为0~25 mg/kg范围内，TRGS和INT I1主要在后肠减少，而在较高TC胁迫下，TRGS和INT I1则有所增加。因此，我们建议生活污泥(DS)中的TC限量为25 mg/kg。尽管优势属在TC胁迫下对TC敏感，但许多含有多个TRG的细菌宿主(特别是后肠中的那些)值得进一步关注。前肠中含有丰富的四环素耐药菌(TRB)的5个属为专化类群。在这些属中，未归类的单胞菌属和小球菌属可能与消化过程有关。与TRGS有关的其他未分类分类群可能来源于DS。肠道共有5个富含TRB的属，后肠共有3个特化属。这些属可将TRGS传播到环境中。这些结果表明，在TC浓度不超过25 mg/kg的DS中，蠕虫堆肥是一种可行的TRG控制方法。TRGS和INI的命运在肠道中有很大的不同，显示出与细菌群落不可避免的联系。
Vermi-composting is considered to be a feasible method for reducing tetracycline resistance genes (TRGs) in the sludge. Nevertheless, the way different gut passages of earthworm might affect the fates of TRGs and whether this process is affected by tetracycline (TC) concentrations need to be further investigated. In this study, we examined the effects of TC concentrations on changes in TRGs and bacterial communities in gut passages of earthworm were determined by using quantitative PCR and Illumina high-throughput sequencing. TRGs andintI1were mainly reduced in the hindgut under the TC concentrations ranging from 0 to 25 mg/kg, while they were enriched under higher TC stress exposure. Consequently, we suggest the TC limitation of 25 mg/kg in the domestic sludge (DS) for vermi-composting. Although the predominant genera were TC sensitive under TC stress, many bacterial hosts harboring multiple TRGs (especially those in the hindgut) should be paid further attention to. In the foregut, five genera with abundant tetracycline-resistant bacteria (TRB) were specialized taxa. Among these genera, Unclassified_Solirubrobacterales andPirellulaceaewere probably related to the digestion processes. Other unclassified taxa related to the TRGs were probably derived from the DS. Five genera with abundant TRB were shared in the gut passages, and three specialized genera in the hindgut. These genera could spread TRGs andintI1to the environment. These results suggest that vermi-composting is a feasible approach for TRG control in the DS containing TC concentration that does not exceed 25 mg/kg. Fates of TRGs andintI1widely differ in the gut passages, showing inevitable connections with bacterial communities.
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影响因子：
15.7
作者：
C. Mougin;N. Cheviron;Cédric Répinçay;M. Hedde;G. Hernandez-Raquet
通讯作者：
C. Mougin;N. Cheviron;Cédric Répinçay;M. Hedde;G. Hernandez-Raquet
影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
12.8
作者：
Ying Yang;Bing Li;S. Zou;H. Fang;Tong Zhang
通讯作者：
Ying Yang;Bing Li;S. Zou;H. Fang;Tong Zhang
影响因子：
--
作者：
SHANNON, CE
通讯作者：
SHANNON, CE
蚯蚓堆肥过程中污泥对四环素和氟喹诺酮抗性基因去向的影响
影响因子：
11.4
作者：
Huang, Kui;Xia, Hui;Wu, Nan
通讯作者：
Wu, Nan