Functional transplantation of salivary gland cells differentiated from mouse early ES cells in vitro.
Functional transplantation of salivary gland cells differentiated from mouse early ES cells in vitro.
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唾液腺细胞的功能移植与小鼠早期ES细胞的体外移植。
DOI：
10.1007/s13577-013-0061-z
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发表时间：
2013-06
期刊：
影响因子：
4.3
通讯作者：
Mataga, Izumi
中科院分区：
文献类型：
--
作者：
Kawakami, Miyuki;Ishikawa, Hiroshi;Tachibana, Toshiaki;Tanaka, Akira;Mataga, Izumi
由于衰老或疾病引起的唾液腺萎缩或功能减退会导致唾液分泌不足，影响患者的生活质量，例如，不仅是口干，而且咀嚼/吞咽障碍的恶化和口腔卫生状况。目前临床上对唾液腺萎缩或功能减退的治疗仅是药物和人工唾液的对症治疗，因此建立根治性治疗是可取的。此时，作为一项基础性研究，我们尝试通过将小鼠早期ES （mEES-6）细胞与人唾液腺源性成纤维细胞（hSG-fibro）共培养，将mEES-6细胞分化为唾液腺细胞。同时，研究了细胞移植的可能性。将转染gfp的mEES-6细胞和hSG-fibro共培养的细胞鉴定后，移植到SCID小鼠颌下腺，观察向组织分化的程度。研究了三维培养系统中共培养细胞组织功能重构的可能性。我们的研究结果证实，共培养细胞表达唾液腺相关标志物，并具有通过体内移植产生新组织的能力。此外，这些细胞可以在三维培养系统中重建腺体结构。通过与hsg -纤维共培养，mEES-6细胞成功分化为可移植的唾液腺细胞，并具有组织新生能力。
Atrophy or hypofunction of the salivary gland because of aging or disease causes hyposalivation and has an effect on the quality of life of patients, for example not only dry mouth but deterioration in mastication/deglutition disorder and the status of oral hygiene. Currently conducted therapies for atrophy or hypofunction of the salivary gland in clinical practice are only symptomatic treatments with drugs and artificial saliva, and therefore it is preferable to establish a radical therapy. At this time, as a fundamental investigation, by co-culturing mouse early ES (mEES-6) cells with human salivary gland-derived fibroblasts (hSG-fibro), differentiation of mEES-6 cells to salivary gland cells has been attempted. Also, the possibility of cell engraftment was examined. After identifying the cells which were co-cultured with GFP-transfected mEES-6 cells and hSG-fibro, the cells were transplanted into the submandibular gland of SCID mice, and the degree of differentiation into tissues was examined. The possibility of tissue functional reconstitution from co-cultured cells in a three-dimensional culture system was examined. Our results confirmed that the co-cultured cells expressed salivary gland-related markers and had an ability to generate neo-tissues by transplantation in vivo. Moreover, the cells could reconstitute gland structures in a three-dimensional culture system. By co-culture with hSG-fibro, mEES-6 cells were successfully differentiated into salivary gland cells which were transplantable and have tissue neogenetic ability.
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干细胞移植在辐照腺体中的干细胞移植后拯救唾液腺功能。
影响因子：
3.7
作者：
Lombaert, Isabelle M. A.;Brunsting, Jeanette F.;Wierenga, Pieter K.;Faber, Hette;Stokman, Monique A.;Kok, Tineke;Visser, Willy H.;Kampinga, Harm H.;de Haan, Gerald;Coppes, Robert P.
通讯作者：
Coppes, Robert P.
影响因子：
64.8
作者：
SMITH, AG;HEATH, JK;ROGERS, D
通讯作者：
ROGERS, D
影响因子：
--
作者：
Man, YG;Ball, WD;Hand, AR
通讯作者：
Hand, AR
影响因子：
3.3
作者：
Sugito, T;Kagami, H;Ueda, M
通讯作者：
Ueda, M
影响因子：
64.8
作者：
WILLIAMS, RL;HILTON, DJ;GOUGH, NM
通讯作者：
GOUGH, NM