DNA-lipid complexes carrying azobenzene moieties: Preparation, characterization, and photoisomerization
DNA-lipid complexes carrying azobenzene moieties: Preparation, characterization, and photoisomerization
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携带偶氮苯部分的 DNA-脂质复合物：制备、表征和光异构化
DOI：
10.1016/j.polymer.2009.09.058
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发表时间：
2009-11
期刊：
影响因子：
4.6
通讯作者：
Masuda, Toshio
中科院分区：
文献类型：
--
作者：
Qu, Jinqing;Qiu, Zhiming;Chen, Huanqin;Ogata, Naoya;Masuda, Toshio
通过用阳离子两亲性脂质（类脂（AZO）和类脂（diAZO））取代钠离子，制备了带有偶氮苯基团的新型DNA-类脂复合物，其中磷酸根与类脂的实际摩尔比分别为1：1.05和1：1.02。DNA-脂质（AZO）和DNA-脂质（diAZO）复合物可溶于常见的有机溶剂，包括CHCl 3、CH 2Cl 2、甲醇和乙醇，而不溶于THF、甲苯和水。CD光谱显示DNA-脂质（AZO）和DNA-脂质（diAZO）复合物在甲醇和CHCl 3中主要呈双螺旋结构，且该螺旋结构对加热相当稳定。紫外光照射后，DNA-脂质复合物侧链中的反式偶氮苯异构化为顺式，而DNA骨架的螺旋构象几乎没有变化。在可见光照射下，顺式偶氮苯部分重新异构化为反式，但它们没有完全恢复侧链中偶氮苯部分的原始几何形状。DNA-脂质（AZO）和DNA-脂质（diAZO）均表现出溶致液晶性质。根据空气中的TGA，DNA-脂质（AZO）和DNA-脂质（diAZO）的重量损失的起始温度均为226°C。
Novel DNA-lipid complexes carrying azobenzene moieties were prepared by substituting sodium counter cations with cationic amphiphilic lipids, namely lipid(AZO) and lipid(diAZO), in which the actual molar ratios of phosphate to lipid were 1:1.05 and 1:1.02, respectively. DNA-lipid(AZO) and DNA-lipid(diAZO) complexes were soluble in common organic solvents including CHCl3, CH2Cl2, methanol, and ethanol, while insoluble in THF, toluene, and water. CD spectroscopy revealed that DNA-lipid(AZO) and DNA-lipid(diAZO) complexes took a predominantly double helical structure in methanol and CHCl3and that the helical structure was fairly stable against heating. The trans-azobenzene of the DNA-lipid complexes in the side chain isomerized into cis upon UV irradiation, while the helical conformation of DNA backbone hardly changed. The cis-azobenzene moiety reisomerized into trans upon visible-light irradiation, but they did not recover completely the original geometry of azobenzene moieties in the side chain. Both DNA-lipid(AZO) and DNA-lipid(diAZO) exhibited lyotropic LC properties. The onset temperatures of weight loss of DNA-lipid(AZO) and DNA-lipid(diAZO) were both 226°C according to TGA in air.
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影响因子：
56.9
作者：
Radler, JO;Koltover, I;Safinya, CR
通讯作者：
Safinya, CR
影响因子：
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作者：
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作者：
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DOI：
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发表时间：
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期刊：
Progress in nucleic acid research and molecular biology
影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/j.ijbiomac.2006.08.016
发表时间：
2007-03
影响因子：
8.2
作者：
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