Colonic pericryptal fibroblasts
Colonic pericryptal fibroblasts
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结肠隐窝周围成纤维细胞
DOI：
10.1007/bf00718649
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
G. Gabbiani
中科院分区：
文献类型：
--
作者：
A. Sappino;P. Dietrich;O. Skalli;S. Widgren;G. Gabbiani
实验证据表明，胎儿肠道间充质细胞可以调节上皮细胞分化。据推测，在消化道中的相互间质-上皮相互作用维持超过胚胎生命。成熟的结肠粘膜含有隐窝周围成纤维细胞（PCF），这是一种表现出平滑肌形态特征的基质细胞类型，被认为调节邻近上皮细胞的生长和分化。使用针对α-平滑肌肌动蛋白（在平滑肌细胞中持续表达）的抗体，我们对人胚胎组织进行了免疫组织化学研究，以评估发育过程中PCF的分化。PCF表达α-平滑肌肌动蛋白，第一次检测到怀孕的第21周左右，在基地的隐窝，分化的PCF的数量逐渐增加，然后与上皮细胞增殖同步，以实现在出生时发现的特征性分布在成人。我们分析了一系列的非恶性和恶性上皮增生性病变的成人结肠相同的技术。在10/10例单纯管状腺瘤中仅观察到稀疏的免疫反应性PCF，而在11/11例绒毛状腺瘤中，免疫反应性PCF始终被发现与增生上皮交界。有趣的是，3/5的乳头状腺瘤，与中度至显著发育不良的区域相关，表现出多层免疫反应PCF的病灶。在检查的14/14的癌症中，PCF不再可识别;表达不同量的β-平滑肌肌动蛋白（构成促结缔组织增生反应）的基质细胞持续存在。这些观察结果建立了化学上的PCF的平滑肌表型特征，其在妊娠中期获得，以及PCF与一些上皮瘤形成一起增殖的能力。这些研究结果可能有助于澄清PCF的组织发生，并提高我们对间充质-上皮相互作用的理解，怀疑在器官发生过程中以及良性和恶性肿瘤的情况下操作。
Experimental evidence has shown that fetal gut mesenchymal cells can modulate epithelial cell differentiation. It is postulated that reciprocal stromal-epithelial interactions in the digestive tract are maintained beyond embryonic life. The mature colonic mucosa contains pericryptal fibroblasts (PCF), a stromal cell type exhibiting smooth muscle morphological features, which are thought to regulate the growth and differentiation of adjacent epithelial cells. Using an antibody directed at α-smooth muscle actin, which is constantly expressed in smooth muscle cells, we performed an immunohistochemical study on human embryonic tissues to assess PCF differentiation during development. PCF expressing ct-smooth muscle actin were first detected around the 21st week of gestation, at the bases of the crypts; the number of differentiated PCF increased then progressively, in synchrony with epithelial proliferation, to achieve at birth the characteristic distribution found in adults. We analyzed a series of non-malignant and malignant epithelial proliferative lesions of the adult colon by the same technique. Only sparse immunoreactive PCF were observed in 10/10 pure tubular adenomas, whereas in 11/11 villous adenomas immunoreactive PCF were consistently found bordering proliferative epithelia. Interestingly, 3/5 papillary adenomas, associated with areas of moderate to marked dysplasia, demonstrated foci of multi-layered immunoreactive PCF. In 14/14 carcinomas examined, PCF were no longer recognizable; stromal cells expressing variable amounts of β-smooth muscle actin, constituting the desmoplastic reaction, were constantly present. These observations establish immunohistochemically a smooth muscle phenotypic feature of PCF, which is acquired at mid-gestation, and the ability of PCF to proliferate in conjunction with some epithelial neoplasias. These findings might help to clarify the histogenesis of PCF and to improve our understanding of the mesenchymal-epithelial interactions suspected to operate during organogenesis as well as benign and malignant neoplastic conditions.
影响因子：
3.6
作者：
GLASSER, SR;JULIAN, J
通讯作者：
JULIAN, J