Fluorescently labeled 1 nm thin nanomembranes.
Fluorescently labeled 1 nm thin nanomembranes.
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荧光标记的 1 nm 薄纳米膜。
DOI：
10.1016/j.jbiotec.2010.01.018
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发表时间：
2010
影响因子：
4.1
通讯作者：
A. Gölzhäuser
中科院分区：
文献类型：
--
作者：
Christoph T. Nottbohm;Ran Sopher;M. Heilemann;M. Sauer;A. Gölzhäuser
自组装单分子膜的荧光标记在化学和生物技术传感方面具有巨大的潜力。然而，它的使用受到在导电衬底附近的荧光猝灭的限制。我们表明，这种淬火可以克服标记的交联芳香SAM（纳米片）和其随后转移到一个非导电基板上。我们证明了成功的标记的纳米片与荧光团（四甲基罗丹明）和其随后的转移到氧化硅，在那里它们被检测到的光学以及荧光显微镜。通过将纳米片类似地转移到TEM网格上来获得双标记的独立纳米片，即纳米膜。
Fluorescent labeling of self-assembled monolayers (SAMs) has a great potential for chemical and biotechnological sensing. However, its use is limited by the quenching of the fluorescence in the proximity of the conducting substrates. We show that this quenching can be overcome by the labeling of a cross-linked aromatic SAM (nanosheet) and its subsequent transfer onto a non-conducting substrate. We demonstrate the successful labeling of nanosheets with a fluorophore (tetramethylrhodamine) and its subsequent transfer to oxidized silicon, where they are detected by optical as well as fluorescence microscopy. Fluorescently labeled freestanding nanosheets, i.e. nanomembranes were obtained by a similar transfer of the nanosheets to TEM grids.
影响因子：
4
作者：
Blake, P.;Hill, E. W.;Geim, A. K.
通讯作者：
Geim, A. K.