Ginsenoside Rb1 attenuates oxygen-glucose deprivation-induced apoptosis in SH-SY5Y cells via protection of mitochondria and inhibition of AIF and cytochrome c release.
Ginsenoside Rb1 attenuates oxygen-glucose deprivation-induced apoptosis in SH-SY5Y cells via protection of mitochondria and inhibition of AIF and cytochrome c release.
复制标题
人参皂苷 Rb1 通过保护线粒体和抑制 AIF 和细胞色素 c 释放来减轻 SH-SY5Y 细胞中缺氧葡萄糖诱导的细胞凋亡
DOI：
10.3390/molecules181012777
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发表时间：
2013-10-16
期刊：
影响因子：
--
通讯作者：
Ge P
中科院分区：
文献类型：
--
作者：
Liang J;Yu Y;Wang B;Lu B;Zhang J;Zhang H;Ge P
为探讨线粒体在人参皂苷Rb 1对缺氧缺糖诱导的细胞凋亡的保护作用中的作用，本研究采用MTT法、TUNEL染色、流式细胞术、免疫细胞化学和Western blotting等方法检测了缺氧缺糖诱导的细胞活力、细胞凋亡、ROS水平、线粒体膜电位以及凋亡诱导因子细胞色素c、Bax和Bcl-2在细胞核中的分布，线粒体和细胞质。我们发现GRb 1预处理改善了OGD损伤的细胞活力。GRb 1还能抑制OGD诱导的SH-SY 5 Y细胞凋亡。进一步的研究表明，GRb 1可以抵消OGD引起的细胞活性氧水平的升高和线粒体膜电位的降低。此外，GRb 1还能抑制凋亡诱导因子向细胞核和细胞色素c向细胞质的移位，并抑制线粒体内Bax的增加和Bcl-2的减少。我们的研究表明，GRb 1对OGD诱导的SH-SY 5 Y细胞凋亡的保护作用与其保护线粒体功能以及抑制AIF和细胞色素c的释放有关。
To investigate the role of mitochondria in the protective effects of ginsenoside Rb1 on cellular apoptosis caused by oxygen-glucose deprivation, in this study, MTT assay, TUNEL staining, flow cytometry, immunocytochemistry and western blotting were used to examine the cellular viability, apoptosis, ROS level, mitochondrial membrane potential, and the distribution of apoptosis inducing factor, cytochrome c, Bax and Bcl-2 in nucleus, mitochondria and cytoplasm. We found that pretreatment with GRb1 improved the cellular viability damaged by OGD. Moreover, GRb1 inhibited apoptosis in SH-SY5Y cells induced by OGD. Further studies showed that the elevation of cellular reactive oxygen species levels and the reduction of mitochondrial membrane potential caused by OGD were both counteracted by GRb1. Additionally, GRb1 not only suppressed the translocation of apoptosis inducing factor into nucleus and cytochrome c into cytoplasm, but also inhibited the increase of Bax within mitochondria and alleviated the decrease of mitochondrial Bcl-2. Our study indicates that the protection of GRb1 on OGD-induced apoptosis in SH-SY5Y cells is associated with its protection on mitochondrial function and inhibition of release of AIF and cytochrome c.
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