Fine Mapping of CsVYL, Conferring Virescent Leaf Through the Regulation of Chloroplast Development in Cucumber.
Fine Mapping of CsVYL, Conferring Virescent Leaf Through the Regulation of Chloroplast Development in Cucumber.
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CsVYL 精细定位，通过调控黄瓜叶绿体发育赋予绿叶
DOI：
10.3389/fpls.2018.00432
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发表时间：
2018
影响因子：
5.6
通讯作者：
Lou Q
中科院分区：
文献类型：
--
作者：
Song M;Wei Q;Wang J;Fu W;Qin X;Lu X;Cheng F;Yang K;Zhang L;Yu X;Li J;Chen J;Lou Q
高等植物叶片颜色突变体是研究光合系统结构和功能的理想材料。在本研究中，我们在突变体文库中发现了一个黄瓜青黄色叶片突变体，该突变体表现出色素含量降低和叶绿体发育过程延迟。利用vyl突变体与黄瓜自交系“Hazerd”杂交构建F2和BC1群体，发现vyl性状由一个简单隐性基因CsVYL控制。利用这80个F2个体和BSA（散装分离分析）方法，将CsVYL基因定位在4号染色体上3.8 cM的区间上。对1542株F2植株进行精细遗传图谱分析，将vyl位点缩小到86.3 kb的基因组区域，共包含11个基因。野生型（WT）与野生型（vyl）的序列比对仅在Csa4G637110基因的第一个外显子上发现了一个单核苷酸突变（C→T），该突变编码dnaj样锌指蛋白。基因表达分析证实了Csa4G637110在野生型和突变型植株间的转录水平差异。CsVYL基因的克隆可以加快黄瓜叶绿体发育和叶绿素合成的研究。
Leaf color mutants in higher plants are ideal materials for investigating the structure and function of photosynthetic system. In this study, we identified a cucumber vyl (virescent-yellow leaf) mutant in the mutant library, which exhibited reduced pigment contents and delayed chloroplast development process. F2 and BC1 populations were constructed from the cross between vyl mutant and cucumber inbred line ‘Hazerd’ to identify that the vyl trait is controlled by a simply recessive gene designated as CsVYL. The CsVYL gene was mapped to a 3.8 cM interval on chromosome 4 using these 80 F2 individuals and BSA (bulked segregation analysis) approach. Fine genetic map was conducted with 1542 F2 plants and narrowed down the vyl locus to an 86.3 kb genomic region, which contains a total of 11 genes. Sequence alignment between the wild type (WT) and vyl only identified one single nucleotide mutation (C→T) in the first exon of gene Csa4G637110, which encodes a DnaJ-like zinc finger protein. Gene Expression analysis confirmed the differences in transcription level of Csa4G637110 between wild type and mutant plants. Map-based cloning of the CsVYL gene could accelerate the study of chloroplast development and chlorophyll synthesis of cucumber.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.1
作者：
HOPKINS, WG;ELFMAN, B
通讯作者：
ELFMAN, B
影响因子：
13.8
作者：
GEORGOPOULOS, C
通讯作者：
GEORGOPOULOS, C
影响因子：
7.4
作者：
Falbel, TG;Meehl, JB;Staehelin, LA
通讯作者：
Staehelin, LA
影响因子：
7.2
作者：
Li, L;Paolillo, DJ;Garvin, DF
通讯作者：
Garvin, DF