Characterization of Calcium-Mediated Intracellular and Intercellular Signaling in the rMC-1 Glial Cell Line.
Characterization of Calcium-Mediated Intracellular and Intercellular Signaling in the rMC-1 Glial Cell Line.
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DOI：
10.1007/s12195-008-0039-1
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发表时间：
2009-03-01
影响因子：
2.8
通讯作者：
Silva, Gabriel A.
中科院分区：
文献类型：
--
作者：
Yu, Diana;Buibas, Marius;Chow, Siu-Kei;Lee, Ian Y.;Singer, Zakary;Silva, Gabriel A.
视网膜Müler神经胶质细胞除了为视网膜神经元提供稳态支持外，还参与调节神经元活动和调节视网膜的血管运动反应等功能。Müller细胞中的钙介导信号在执行这些功能所需的细胞内和细胞间相互作用中发挥着重要作用。虽然Müler细胞内钙信号的基本分子机制已经被描述，但Müler细胞内钙反应的动力学还没有得到充分的研究。在这里，我们使用RMC-1细胞系(一种由大鼠Müler细胞发展而来的成熟系)，在Müller细胞信号转导的体外模型中提供了钙信号的定量表征。RMC-1细胞表现出强大的细胞内钙瞬变和支持钙瞬变介导的细胞间钙波的能力，其信号动力学类似于在原位视网膜准备中报道的Müler细胞。此外，thapsigargin对细胞内钙瞬变和嘌呤能受体拮抗剂和缝隙连接阻滞剂(分别为PPADS和FFA)对细胞间钙波的药理学扰动表明，RMC-1细胞钙信号的分子机制与Müller细胞的机制是保守的。该模型为研究Müler细胞内和细胞间钙信号的特定机制假说提供了一个可靠的体外系统。
Retinal Müller glial cells, in addition to providing homeostatic support to retinal neurons, have been shown to engage in modulation of neuronal activity and regulate vasomotor responses in the retina, among other functions. Calcium-mediated signaling in Müller cells has been implicated to play a significant role in the intracellular and intercellular interactions necessary to carry out these functions. Although the basic molecular mechanisms of calcium signaling in Müller cells have been described, the dynamics of calcium responses in Müller cells have not been fully explored. Here, we provide a quantitative characterization of calcium signaling in an in vitro model of Müller cell signaling using the rMC-1 cell line, a well-established line developed from rat Müller cells. rMC-1 cells displayed robust intracellular calcium transients and the capacity to support calcium transient-mediated intercellular calcium waves with signaling dynamics similar to that reported for Müller cells in in situ retinal preparations. Furthermore, pharmacological perturbations of intracellular calcium transients with thapsigargin and intercellular calcium waves with purinergic receptor antagonists and gap junction blockers (PPADS and FFA, respectively) suggest that the molecular mechanisms that underlie calcium signaling in rMC-1 cells has been conserved with those of Müller cells. This model provides a robust in vitro system for investigating specific mechanistic hypotheses of intra- and intercellular calcium signaling in Müller cells.
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作者：
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通讯作者：
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