Cortical domain correction repositions the polarity boundary to match the cytokinesis furrow in C. elegans embryos
Cortical domain correction repositions the polarity boundary to match the cytokinesis furrow in C. elegans embryos
复制标题
皮质域校正重新定位极性边界以匹配线虫胚胎中的胞质分裂沟
作者：
Christian Schenk;H. Bringmann;A. Hyman;C. Cowan
在不对称分裂的细胞中，不能协调细胞极性与细胞分裂位点可导致细胞命运转变和肿瘤发生。细胞极性在C.线虫胚胎由PAR蛋白定义，其占据细胞皮层的倒数两半。在不对称分裂过程中，前和后PAR结构域之间的边界精确地匹配细胞分裂的位点，确保细胞命运的排他性分离。PAR结构域通过定位有丝分裂纺锤体来确定细胞分裂的位点，这表明细胞极性和细胞分裂可能是协调的一种手段。在这里，我们报告说，细胞极性和细胞分裂协调通过一个额外的机制：细胞分裂的网站重新定位PAR-2的边界。Gα介导的微管-皮质相互作用似乎将PAR-2和肌球蛋白的皮质流导向细胞分裂位点，其充当PAR-2和肌球蛋白的汇。PAR-2边界校正缺陷的胚胎发生皮层极性和细胞质决定因素的错误分离，这表明PAR结构域校正可能有助于防止细胞命运的转变。
In asymmetrically dividing cells, a failure to coordinate cell polarity with the site of cell division can lead to cell fate transformations and tumorigenesis. Cell polarity in C. elegans embryos is defined by PAR proteins, which occupy reciprocal halves of the cell cortex. During asymmetric division, the boundary between the anterior and posterior PAR domains precisely matches the site of cell division, ensuring exclusive segregation of cell fate. The PAR domains determine the site of cell division by positioning the mitotic spindle, suggesting one means by which cell polarity and cell division might be coordinated. Here, we report that cell polarity and cell division are coordinated through an additional mechanism: the site of cell division repositions the PAR-2 boundary. Gα-mediated microtubule-cortex interactions appear to direct cortical flows of PAR-2 and myosin toward the site of cell division, which acts as a PAR-2 and myosin sink. Embryos with defects in PAR-2 boundary correction undergo mis-segregation of cortical polarity and cytoplasmic determinants, suggesting that PAR domain correction might help prevent cell fate transformation.
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影响因子：
64.5
作者：
K. Sanada;L. Tsai
通讯作者：
K. Sanada;L. Tsai
影响因子：
3.3
作者：
Raich, WB;Moran, AN;Hardin, J
通讯作者：
Hardin, J
影响因子：
11.4
作者：
Malerod, Lene;Le Borgne, Roland;Haglund, Kaisa
通讯作者：
Haglund, Kaisa
DOI：
10.1895/wormbook.1.9.1
发表时间：
2005-07-28
期刊：
WormBook : the online review of C. elegans biology
影响因子：
--
作者：
Strome, Susan
通讯作者：
Strome, Susan