Stable, small, specific, low-valency quantum dots for single-molecule imaging.
Stable, small, specific, low-valency quantum dots for single-molecule imaging.
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DOI：
10.1039/c7nr08673c
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发表时间：
2018-03-01
期刊：
影响因子：
6.7
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Lee J ;Feng X ;Chen O ;Bawendi MG ;Huang J
We developed a strategy for synthesizing immediately activable, water-soluble, compact (~10-12 nm hydrodynamic diameter) quantum dots with a small number of stable and controllable conjugation handles for long distance delivery and subsequent biomolecule conjugation. Upon covalent conjugation with engineered monovalent streptavidin, the sample results in a population consisting of low-valency quantum dots. Alternatively, we synthesized a quantum dot with a small number of biotin molecules that can self-assemble with engineered divalent streptavidin via high-affinity biotin-streptavidin interaction. Compact, stable and highly specific against biotinylated proteins of interest, these low-valency quantum dots are ideal for labeling and tracking single molecules on the cell surface with high spatiotemporal resolution for different biological systems and applications.
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