Sequencing our way towards understanding global eukaryotic biodiversity.

Sequencing our way towards understanding global eukaryotic biodiversity.
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DOI:
10.1016/j.tree.2011.11.010
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发表时间:
2012-04
影响因子:
16.8
通讯作者:
Thomas WK
Thomas WK
中科院分区:
生物学1区
文献类型:
--
作者:
Bik HM;Porazinska DL;Creer S;Caporaso JG;Knight R;Thomas WK

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微观真核生物是丰富的,多样的,并填补了地球上每个生态系统的关键生态角色,但在我们对其全球生物多样性的理解中存在着一个公认的差距。DNA测序和生物信息学的基本进展现在允许从环境样品中精确地对微观真核生物的生物多样性进行评估。尽管前景看好,但真核生物标记基因调查领域面临着重大挑战:如何生成对社区最有用的数据,特别是在面对不断发展的测序技术和生物信息学管道时,以及如何纳入越来越多的靶基因。
Microscopic eukaryotes are abundant, diverse, and fill critical ecological roles across every ecosystem on earth, yet there is a well-recognized gap in our understanding of their global biodiversity. Fundamental advances in DNA sequencing and bioinformatics now allow accurate en masse biodiversity assessments of microscopic eukaryotes from environmental samples. Despite a promising outlook, the field of eukaryotic marker gene surveys faces significant challenges: how to generate data that is most useful to the community, especially in the face of evolving sequencing technology and bioinformatics pipelines, and how to incorporate an expanding number of target genes.
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