Novel insights into the genetic basis of buffalo reproductive performance.
Novel insights into the genetic basis of buffalo reproductive performance.
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对水牛繁殖性能遗传基础的新见解
DOI：
10.1186/s12864-018-5208-6
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发表时间：
2018-11-12
期刊：
影响因子：
4.4
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Li J;Liu J;Campanile G;Plastow G;Zhang C;Wang Z;Cassandro M;Gasparrini B;Salzano A;Hua G;Liang A;Yang L
生育力是一种复杂的性状，对水牛产业的发展有重大影响。全基因组关联研究（GWAS）提高了检测影响复杂性状的基因的能力，在反刍动物中已经鉴定出许多与生殖性状相关的重要基因。然而，生殖性状受多种因素的影响。卵泡的发育是影响生育的最重要的内部过程之一。GWAS发现的与卵泡发育相关的基因可能直接影响生育能力。本研究将GWAS和卵泡颗粒细胞RNA-seq相结合，以鉴定可能影响水牛生育能力的重要基因。结果使用90 K Affymetrix Axiom水牛SNP阵列鉴定了与生殖性状相关的SNP、基因组区域和基因。共鉴定出40个暗示性基因座（涉及28个基因），与6个生殖性状（第一、第二和第三产犊年龄、产犊间隔、每次受胎次数和开放天数）相关。有趣的是，在水牛卵泡颗粒细胞中也观察到其中25个基因的mRNA表达。igfbp7基因在整个窦卵泡生长过程中高水平表达。下调水牛颗粒细胞igfbp7可促进细胞凋亡，抑制细胞增殖，增加孕酮和雌二醇的分泌。此外，在2.3 ~ 2.7 Mb的牛5号染色体上检测到与第二次产犊年龄、产犊间隔和开放日相关的显著信号。结论本研究发现的水牛生殖性状相关基因可能通过调节卵泡生长而影响其生育能力。这些结果可能对通过基因组信息的应用改善水牛育种计划具有重要意义。
BackgroundFertility is a complex trait that has a major impact on the development of the buffalo industry. Genome-wide association study (GWAS) has increased the ability to detect genes influencing complex traits, and many important genes related to reproductive traits have been identified in ruminants. However, reproductive traits are influenced by many factors. The development of the follicle is one of the most important internal processes affecting fertility. Genes found by GWAS to be associated with follicular development may directly affect fertility. The present study combined GWAS and RNA-seq of follicular granulosa cells to identify important genes which may affect fertility in the buffalo.ResultsThe 90 K Affymetrix Axiom Buffalo SNP Array was used to identify the SNPs, genomic regions, and genes that were associated with reproductive traits. A total of 40 suggestive loci (related to 28 genes) were identified to be associated with six reproductive traits (first, second and third calving age, calving interval, the number of services per conception and open days). Interestingly, the mRNA expressions of 25 of these genes were also observed in buffalo follicular granulosa cells. TheIGFBP7gene showed high level of expression during whole antral follicle growth. The knockdown ofIGFBP7in buffalo granulosa cells promoted cell apoptosis and hindered cell proliferation, and increased the production of progesterone and estradiol. Furthermore, a notable signal was detected at 2.3–2.7 Mb on the equivalent of bovine chromosome 5 associated with age at second calving, calving interval, and open days.ConclusionsThe genes associated with buffalo reproductive traits in this study may have effect on fertility by regulating of follicular growth. These results may have important implications for improving buffalo breeding programs through application of genomic information.
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影响因子：
3.8
作者：
Khaitan D;Sankpal UT;Weksler B;Meister EA;Romero IA;Couraud PO;Ningaraj NS
通讯作者：
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作者：
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通讯作者：
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作者：
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通讯作者：
Matzuk, MM