Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer.
Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer.
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DOI：
10.1261/rna.078444.120
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Cayota A
中科院分区：
文献类型：
--
作者：
Tosar JP;García-Silva MR;Cayota A
癌症研究人员对Piwi相互作用RNA（piRNA）的研究兴趣日益增加，piRNA是一类对维持生殖系基因组稳定性非常重要的小分子RNA。piRNA在癌症中的异常表达可能意味着这些调节性RNA参与了肿瘤转化。此外，由于piRNA在健康个体的血液中不应广泛循环，这可能使基于液体活检中的RNA分析进行早期癌症诊断成为可能。事实上，最近已经表明血清piR - 54265能够很好地区分结直肠癌患者和健康对照。然而，我们也已经表明，迄今为止在哺乳动物中报道的大多数体细胞piRNA实际上是其他非编码RNA的片段。在此，我们表明将piR - 54265定位为结直肠癌的一种非侵入性生物标志物的研究实际上是在测量血清中一种全长（72个核苷酸）小核仁RNA水平的变化。这应该为未来体细胞和癌症特异性piRNA的研究敲响警钟。我们大力鼓励这方面的研究，但也讨论了在不受piRNA数据库中错误条目干扰的情况下识别体细胞piRNA的正确方法。我们还引入了杂项piRNA（m - piRNA）的概念，以区分经典piRNA和在体细胞中偶然与PIWI蛋白相关的其他小分子RNA。
There is increasing interest among cancer researchers in the study of Piwi-interacting RNAs (piRNAs), a group of small RNAs important for maintaining genome stability in the germline. Aberrant expression of piRNAs in cancer could imply an involvement of these regulatory RNAs in neoplastic transformation. On top of that, it could enable early cancer diagnosis based on RNA analysis in liquid biopsies, as piRNAs are not expected to widely circulate in the bloodstream of healthy individuals. Indeed, it has recently been shown that serum piR-54265 allows for excellent discrimination between colorectal cancer patients and healthy controls. However, we have also shown that most somatic piRNAs reported to date in mammals are actually fragments of other noncoding RNAs. Herein, we show that reports positioning piR-54265 as a noninvasive biomarker for colorectal cancer were actually measuring variations in the levels of a full-length (72 nt) small nucleolar RNA in serum. This should place a cautionary note for future research in somatic and cancer-specific piRNAs. We deeply encourage this line of research but discuss proper ways to identify somatic piRNAs without the interference of erroneous entries contained in piRNA databases. We also introduce the concept of miscellaneous-piRNAs (m-piRNAs) to distinguish between canonical piRNAs and other small RNAs circumstantially associated with PIWI proteins in somatic cells.
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影响因子：
13.8
作者：
Czech B;Hannon GJ
通讯作者：
Hannon GJ
PIWIL1作为后期促进复合物/环体驱动胰腺癌转移的共激活剂的独立于piRNA的功能
影响因子：
21.3
作者：
Li, Feng;Yuan, Peng;Liu, Mo-Fang
通讯作者：
Liu, Mo-Fang
影响因子：
16.6
作者：
Freedman JE;Gerstein M;Mick E;Rozowsky J;Levy D;Kitchen R;Das S;Shah R;Danielson K;Beaulieu L;Navarro FC;Wang Y;Galeev TR;Holman A;Kwong RY;Murthy V;Tanriverdi SE;Koupenova-Zamor M;Mikhalev E;Tanriverdi K
通讯作者：
Tanriverdi K
影响因子：
64.5
作者：
Elkayam E;Kuhn CD;Tocilj A;Haase AD;Greene EM;Hannon GJ;Joshua-Tor L
通讯作者：
Joshua-Tor L
影响因子：
9.2
作者：
Horwich, Michael D.;Li, Chengjian;Zamore, Phillip D.
通讯作者：
Zamore, Phillip D.