Methods for Improving Aptamer Binding Affinity.
Methods for Improving Aptamer Binding Affinity.
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DOI：
10.3390/molecules21040421
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发表时间：
2016-03-28
期刊：
影响因子：
--
通讯作者：
Ikebukuro K
中科院分区：
文献类型：
--
作者：
Hasegawa H;Savory N;Abe K;Ikebukuro K
适体是结合多种生物靶标的单链寡核苷酸。尽管可以使用基于亲和力的选择从随机序列寡核苷酸池中分离适体，但并不总是获得具有高亲和力的适体。因此，需要进一步细化适体以实现所需的结合亲和力。一级序列的优化和适配体构象的稳定是改善适配体结合特性的主要途径。特别是，结合计算机序列重组和体外功能评估的序列优化对于提高结合亲和力是有效的，然而，适体的结合亲和力受到核酸的低疏水性的限制。因此，将疏水部分引入适体中扩大了适体和靶标之间相互作用的多样性。此外，通过连接识别不同表位的适体来构建多价适体是显着增加结合亲和力的一种有吸引力的方法。此外，可以通过优化多价构建体的支架来调整结合亲和力。在这篇综述中，我们总结了提高适体结合亲和力的各种技术。
Aptamers are single stranded oligonucleotides that bind a wide range of biological targets. Although aptamers can be isolated from pools of random sequence oligonucleotides using affinity-based selection, aptamers with high affinities are not always obtained. Therefore, further refinement of aptamers is required to achieve desired binding affinities. The optimization of primary sequences and stabilization of aptamer conformations are the main approaches to refining the binding properties of aptamers. In particular, sequence optimization using combined in silico sequence recombinations and in vitro functional evaluations is effective for the improvement of binding affinities, however, the binding affinities of aptamers are limited by the low hydrophobicity of nucleic acids. Accordingly, introduction of hydrophobic moieties into aptamers expands the diversity of interactions between aptamers and targets. Moreover, construction of multivalent aptamers by connecting aptamers that recognize distinct epitopes is an attractive approach to substantial increases in binding affinity. In addition, binding affinities can be tuned by optimizing the scaffolds of multivalent constructs. In this review, we summarize the various techniques for improving the binding affinities of aptamers.
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影响因子：
7.4
作者：
Cho, Minseon;Oh, Seung Soo;Nie, Jeff;Stewart, Ron;Radeke, Monte J.;Eisenstein, Michael;Coffey, Peter J.;Thomson, James A.;Soh, H. Tom
通讯作者：
Soh, H. Tom
影响因子：
3.7
作者：
Gold L;Ayers D;Bertino J;Bock C;Bock A;Brody EN;Carter J;Dalby AB;Eaton BE;Fitzwater T;Flather D;Forbes A;Foreman T;Fowler C;Gawande B;Goss M;Gunn M;Gupta S;Halladay D;Heil J;Heilig J;Hicke B;Husar G;Janjic N;Jarvis T;Jennings S;Katilius E;Keeney TR;Kim N;Koch TH;Kraemer S;Kroiss L;Le N;Levine D;Lindsey W;Lollo B;Mayfield W;Mehan M;Mehler R;Nelson SK;Nelson M;Nieuwlandt D;Nikrad M;Ochsner U;Ostroff RM;Otis M;Parker T;Pietrasiewicz S;Resnicow DI;Rohloff J;Sanders G;Sattin S;Schneider D;Singer B;Stanton M;Sterkel A;Stewart A;Stratford S;Vaught JD;Vrkljan M;Walker JJ;Watrobka M;Waugh S;Weiss A;Wilcox SK;Wolfson A;Wolk SK;Zhang C;Zichi D
通讯作者：
Zichi D
DOI：
10.3390/s8021090
发表时间：
2008-02-19
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Hasegawa H;Taira KI;Sode K;Ikebukuro K
通讯作者：
Ikebukuro K
影响因子：
14.9
作者：
Ahmad KM;Xiao Y;Soh HT
通讯作者：
Soh HT
影响因子：
4.1
作者：
Fukaya, Takahiro;Abe, Koichi;Ikebukuro, Kazunori
通讯作者：
Ikebukuro, Kazunori