Monocytic myeloid-derived suppressor cells generated from rhesus macaque bone marrow enrich for regulatory T cells.
Monocytic myeloid-derived suppressor cells generated from rhesus macaque bone marrow enrich for regulatory T cells.
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DOI：
10.1016/j.cellimm.2018.04.013
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发表时间：
2018-07
影响因子：
4.3
通讯作者：
Thomson AW
中科院分区：
文献类型：
--
作者：
Zahorchak AF;Perez-Gutierrez A;Ezzelarab MB;Thomson AW
从正常恒河猴骨髓(BM)细胞经GM-−和IL-6培养7天后，获得了可能的单核细胞来源的髓系抑制细胞(MMDSC；Lineage HLADR−/lo)。根据CD14、CD33、CD34和CD11b的表达差异，确定了三个亚群。Flow分选后，对这些亚群抑制抗CD3/CD28刺激的CD_4和CD_8T细胞增殖的能力的评估表明，最有效的群体是CD14hiCD33−/loCD34loCD11bhi。这些骨髓来源的mMDSC显著增加应答T细胞群中−+CD25+CD127Foxp3+调节性T细胞的发生率。它们在检测免疫调节性mMDSC促进非人类灵长类移植耐受的疗效方面提供了潜在的价值。
Putative monocytic myeloid-derived suppressor cells (mMDSC; lineage−HLA-DR−/lo) were generated in 7-day cultures from normal rhesus macaque bone marrow (BM) cells in GM-CSF and IL-6. Three subsets were identified based on their differential expression of CD14, CD33, CD34 and CD11b. Following flow sorting, assessment of the capacity of these subsets to suppress anti-CD3/CD28-stimulated CD4 and CD8 T cell proliferation revealed that the most potent population was CD14hiCD33−/loCD34loCD11bhi. These BM-derived mMDSC markedly increased the incidence of CD4+CD25+CD127−Foxp3+ regulatory T cells in responder T cell populations. They offer potential value in testing the therapeutic efficacy of immunoregulatory mMDSC for the promotion of tolerance in nonhuman primate transplant models.
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影响因子：
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作者：
Zhang, H.;Guo, H.;Thomson, A. W.
通讯作者：
Thomson, A. W.
影响因子：
20.3
作者：
Movahedi, Kiavash;Guilliams, Martin;Van Ginderachter, Jo A.
通讯作者：
Van Ginderachter, Jo A.
影响因子：
20.3
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DOI：
10.1111/ajt.12310
发表时间：
2013-08
期刊：
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
影响因子：
--
作者：
Ezzelarab MB;Zahorchak AF;Lu L;Morelli AE;Chalasani G;Demetris AJ;Lakkis FG;Wijkstrom M;Murase N;Humar A;Shapiro R;Cooper DK;Thomson AW
通讯作者：
Thomson AW
DOI：
10.1111/ajt.13454
发表时间：
2016-02
期刊：
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
影响因子：
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作者：
Zahorchak AF;Ezzelarab MB;Lu L;Turnquist HR;Thomson AW
通讯作者：
Thomson AW