In Vivo Mobilization and Functional Characterization of Nonhuman Primate Monocytic Myeloid-Derived Suppressor Cells.
In Vivo Mobilization and Functional Characterization of Nonhuman Primate Monocytic Myeloid-Derived Suppressor Cells.
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DOI：
10.1111/ajt.13454
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Thomson AW
中科院分区：
文献类型：
--
作者：
Zahorchak AF;Ezzelarab MB;Lu L;Turnquist HR;Thomson AW
越来越多的来自小动物模型的证据表明，髓源性抑制细胞（MDSC）可以在抑制同种异体移植排斥反应和促进移植耐受中发挥关键作用。在这里，我们鉴定了正常恒河猴外周血中的CD 3 − CD 20 −HLA-DR− CD 14 + CD 33 + CD 11b+细胞。这些推定的单核细胞MDSC占lin−HLA-DR− PBMC的2.1 ± 1.7%。粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞（G）-CSF的给药使其发生率增加至5.3 ± 3.4%。可从单个全恒河猴白细胞去除产物中流式分选的MDSC总数为38 ± 13.106（n=10只猴）。动员猴新鲜分离或冻存的MDSC加入抗CD 3 −和抗CD 28刺激的自体T细胞培养物中，可显著抑制CD 4+和CD 8 + T细胞增殖和细胞因子分泌（IFNγ、IL-17 A）。此外，这些MDSC增强了CD 4 + CD 25 hiFoxp 3+调节性T细胞（Treg）扩增，同时抑制活化的记忆T细胞的增殖并相对于效应细胞和终末分化的记忆T细胞增加Treg。精氨酸酶-1（Arg-1）的抑制，而不是诱导型一氧化氮合酶的活性，部分逆转了MDSC对CD 8 + T细胞增殖的抑制作用。因此，可以从NHP中分离功能性MDSC，用于在移植中作为治疗性细胞疫苗的前瞻性用途。
There is growing evidence from small animal models that myeloid-derived suppressor cells (MDSC) can play a crucial role in inhibiting allograft rejection and in promoting transplant tolerance. Here, we identified CD3−CD20−HLA-DR−CD14+CD33+CD11b+ cells in peripheral blood of normal rhesus macaques. These putative, monocytic MDSC constituted 2.1 ± 1.7% of lin−HLA-DR− PBMC. Administration of granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte (G)-CSF increased their incidence to 5.3 ± 3.4%. The total number of MDSC that could be flow-sorted from a single whole rhesus leukapheresis product was 38 ± 13.106 (n=10 monkeys). Freshly-isolated or cryopreserved MDSC from mobilized monkeys incorporated in cultures of anti-CD3− and anti-CD28-stimulated autologous T cells, markedly suppressed CD4+ and CD8+ T cell proliferation and cytokine secretion (IFNγ, IL-17A). Moreover, these MDSC enhanced CD4+CD25hiFoxp3+ regulatory T cell (Treg) expansion, while inhibiting proliferation of activated memory T cells and increasing Treg relative to effector and terminally-differentiated memory T cells. Inhibition of arginase-1 (Arg-1), but not inducible nitric oxide synthase activity, partially reversed the inhibitory effect of the MDSC on CD8+ T cell proliferation. Thus, functional MDSC can be isolated from NHP for prospective use as therapeutic cellular vaccines in transplantation.
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影响因子：
5.6
作者：
Greten TF;Manns MP;Korangy F
通讯作者：
Korangy F
影响因子：
4.4
作者：
Dilek, Nahzli;Poirier, Nicolas;Vanhove, Bernard
通讯作者：
Vanhove, Bernard
影响因子：
20.3
作者：
Highfill, Steven L.;Rodriguez, Paulo C.;Blazar, Bruce R.
通讯作者：
Blazar, Bruce R.
调节性树突状细胞输注延长非人类灵长类动物的肾脏同种异体移植存活。
DOI：
10.1111/ajt.12310
发表时间：
2013-08
期刊：
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
影响因子：
--
作者：
Ezzelarab MB;Zahorchak AF;Lu L;Morelli AE;Chalasani G;Demetris AJ;Lakkis FG;Wijkstrom M;Murase N;Humar A;Shapiro R;Cooper DK;Thomson AW
通讯作者：
Thomson AW
影响因子：
2.7
作者：
Boros, Peter;Ochando, Jordi C.;Chen, Shu-Hsia;Bromberg, Jonathan S.
通讯作者：
Bromberg, Jonathan S.