Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment.
Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment.
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DOI：
10.18063/ijb.v7i4.444
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发表时间：
2021
影响因子：
8.4
通讯作者：
He M
中科院分区：
文献类型：
--
作者：
Zhuang P;Chiang YH;Fernanda MS;He M
癌症仍然是世界范围内死亡的主要原因。尽管在抗癌治疗方面已经做出了相当大的努力，但进展仍然缓慢，部分原因是缺乏可靠的预测模型。多细胞肿瘤球体作为反映非血管性肿瘤特征的主要三维培养模型，在病理生理学研究和高通量药物筛选中得到了广泛的应用。然而，对细胞和结构组织的有限控制仍然是实现体内类似组织微环境的关键挑战。3D生物打印因其结合细胞和材料的卓越空间控制、可伸缩性和可重复性，在组织/器官模拟方面取得了长足的进步。展望未来，利用3D生物打印和多细胞球体的力量可能会产生更可靠的肿瘤模型，并促进我们对肿瘤进展机制的理解。在这篇综述中，使用球体作为三维生物打印的构建块用于肿瘤建模的新概念被阐明。我们首先描述肿瘤微环境的背景，然后介绍肿瘤球体形成的各种方法，以及它们的具体优点和缺点。之后，我们对现有的使用球体作为焦点的3D打印肿瘤模型进行了概述。我们提供了当代文献来源的汇编，并总结了在3D打印组织建模中使用球体作为构建块的技术和可能性的总体进步，并特别强调了肿瘤模型。对集成3D球体打印的未来发展进行了展望，总结了这一综述。
Cancer still ranks as a leading cause of mortality worldwide. Although considerable efforts have been dedicated to anticancer therapeutics, progress is still slow, partially due to the absence of robust prediction models. Multicellular tumor spheroids, as a major three-dimensional (3D) culture model exhibiting features of avascular tumors, gained great popularity in pathophysiological studies and high throughput drug screening. However, limited control over cellular and structural organization is still the key challenge in achieving in vivo like tissue microenvironment. 3D bioprinting has made great strides toward tissue/organ mimicry, due to its outstanding spatial control through combining both cells and materials, scalability, and reproducibility. Prospectively, harnessing the power from both 3D bioprinting and multicellular spheroids would likely generate more faithful tumor models and advance our understanding on the mechanism of tumor progression. In this review, the emerging concept on using spheroids as a building block in 3D bioprinting for tumor modeling is illustrated. We begin by describing the context of the tumor microenvironment, followed by an introduction of various methodologies for tumor spheroid formation, with their specific merits and drawbacks. Thereafter, we present an overview of existing 3D printed tumor models using spheroids as a focus. We provide a compilation of the contemporary literature sources and summarize the overall advancements in technology and possibilities of using spheroids as building blocks in 3D printed tissue modeling, with a particular emphasis on tumor models. Future outlooks about the wonderous advancements of integrated 3D spheroidal printing conclude this review.
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