Modular polymer design to regulate phenotype and oxidative response of human coronary artery cells for potential stent coating applications.
Modular polymer design to regulate phenotype and oxidative response of human coronary artery cells for potential stent coating applications.
复制标题
DOI：
10.1016/j.actbio.2011.10.003
复制
发表时间：
2012-02
影响因子：
9.7
通讯作者：
Sung, Hak-Joon
中科院分区：
文献类型：
--
作者：
Crowder, Spencer W.;Gupta, Mukesh K.;Hofmeister, Lucas H.;Zachman, Angela L.;Sung, Hak-Joon
聚合物性能可以通过共聚具有特定物理化学特性的亚基来定制。血管支架材料需要具有生物相容性、机械强度和防止再狭窄。在这里，我们以不同的摩尔比共聚聚（ε-己内酯）（PCL）、聚（乙二醇）（PEG）和羧基-PCL（cPCL），并表征了所得材料的性能。然后，我们对这些聚合物作为潜在冠状动脉支架涂层材料的适用性进行了短期评估，其中包括两种主要的人类冠状动脉细胞类型：平滑肌细胞（HCASMC）和内皮细胞（HCAEC）。增殖和表型的变化取决于细胞内活性氧 (ROS) 水平，4%PEG-96%PCL-0%cPCL 被确定为最适合该应用的涂层材料。在这种基质上三天后，HCASMC 保持健康的收缩表型，并且 HCAEC 表现出生理相关的增殖率和平衡的氧化还原状态。其他测试底物促进 HCASMC 中的病理性合成表型和/或 HCAEC 中的过度增殖。 HCASMC 的表型变化似乎受到测试基质的杨氏模量和表面电荷的调节，表明结构-功能关系可用于对血管细胞功能的复杂控制。这些数据表明，定制的共聚物特性可以指导血管细胞行为，并为进一步开发生物指导性支架涂层材料提供见解。
Polymer properties can be tailored by copolymerizing subunits with specific physicochemical characteristics. Vascular stent materials require biocompatibility, mechanical strength, and prevention of restenosis. Here we copolymerized poly(ε-caprolactone) (PCL), poly(ethylene glycol) (PEG), and carboxyl-PCL (cPCL) at varying molar ratios and characterized the resulting material properties. We then performed a short-term evaluation of these polymers for their applicability as potential coronary stent coating materials with two primary human coronary artery cell types: smooth muscle cells (HCASMCs) and endothelial cells (HCAECs). Changes in proliferation and phenotype were dependent upon intracellular reactive oxygen species (ROS) levels, and 4%PEG-96%PCL-0%cPCL was identified as the most appropriate coating material for this application. After three days on this substrate, HCASMCs maintained a healthy contractile phenotype and HCAECs exhibited a physiologically-relevant proliferation rate and a balanced redox state. Other test substrates promoted a pathological, synthetic phenotype in HCASMCs and/or hyperproliferation in HCAECs. Phenotypic changes of HCASMCs appeared to be modulated by Young’s modulus and surface charge of test substrates, indicating a structure-function relationship that can be exploited for intricate control over vascular cell functions. These data indicate that tailored copolymer properties can direct vascular cell behavior and provide insight for further development of biologically instructive stent coating materials.
登录
查看更多内容
影响因子：
3.9
作者：
Kwok, DY;Gietzelt, T;Neumann, AW
通讯作者：
Neumann, AW
影响因子：
3.9
作者：
Roy, Dhruvajyoti;Kwak, Ju-Won;Park, Joon Won
通讯作者：
Park, Joon Won
影响因子：
1.7
作者：
Gimenez, S;Ponsart, S;Vert, M
通讯作者：
Vert, M
影响因子：
37.8
作者：
Khatri, JJ;Johnson, C;Galis, ZS
通讯作者：
Galis, ZS
影响因子：
3.8
作者：
Sung, HJ;Eskin, SG;McIntire, LV
通讯作者：
McIntire, LV