Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells.
Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells.
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DOI：
10.1038/ncomms7810
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发表时间：
2015-04-10
影响因子：
16.6
通讯作者：
Baek, Sung Hee
中科院分区：
文献类型：
--
作者：
Boo, Kyungjin;Bhin, Jinhyuk;Jeon, Yoon;Kim, Joomyung;Shin, Hi-Jai R.;Park, Jong-Eun;Kim, Kyeongkyu;Kim, Chang Rok;Jang, Hyonchol;Kim, In-Hoo;Kim, V. Narry;Hwang, Daehee;Lee, Ho;Baek, Sung Hee
转录因子、染色质修饰剂和非编码RNA的作用对于细胞状态的编程至关重要。虽然各种表观遗传机制控制胚胎干细胞（ES）的多能性的重要性已被研究，染色质修饰剂如何与特定的转录因子合作仍然在很大程度上难以捉摸。在这里，我们发现桥蛋白染色质重塑因子作为Oct 4的共激活因子在小鼠ES细胞中维持多能性中起着重要作用。全基因组分析显示，Pontin和Oct 4共享一组参与ES细胞维持的靶基因。有趣的是，我们发现桥蛋白的Oct 4依赖性辅激活因子功能延伸到大的基因间非编码RNA（lincRNA）的转录，特别是linc 1253，一种抑制lincRNA的谱系程序，是桥蛋白依赖性Oct 4靶向lincRNA。总之，我们的研究结果表明，Oct 4-Pontin模块在调控参与ES细胞命运决定的基因中起着关键作用。 在胚胎干（ES）细胞中鉴定的长非编码RNA或lincRNA已显示出调节ES细胞状态;然而，这些lincRNA如何被调节仍不清楚。在这里，作者表明转录辅激活因子Pontin以Oct 4依赖性方式调节参与ES细胞维持的lincRNA的表达。
The actions of transcription factors, chromatin modifiers and noncoding RNAs are crucial for the programming of cell states. Although the importance of various epigenetic machineries for controlling pluripotency of embryonic stem (ES) cells has been previously studied, how chromatin modifiers cooperate with specific transcription factors still remains largely elusive. Here, we find that Pontin chromatin remodelling factor plays an essential role as a coactivator for Oct4 for maintenance of pluripotency in mouse ES cells. Genome-wide analyses reveal that Pontin and Oct4 share a substantial set of target genes involved in ES cell maintenance. Intriguingly, we find that the Oct4-dependent coactivator function of Pontin extends to the transcription of large intergenic noncoding RNAs (lincRNAs) and in particular linc1253, a lineage programme repressing lincRNA, is a Pontin-dependent Oct4 target lincRNA. Together, our findings demonstrate that the Oct4-Pontin module plays critical roles in the regulation of genes involved in ES cell fate determination. Long non-coding RNAs or lincRNAs identified in embryonic stem (ES) cells have been shown to regulate ES cell states; however, how these lincRNAs are regulated remains unclear. Here the authors show that the transcriptional coactivator Pontin regulates the expression of lincRNAs involved in ES cell maintenance in an Oct4-dependent manner.
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影响因子：
4
作者：
Flynn, Ryan A.;Chang, Howard Y.
通讯作者：
Chang, Howard Y.
影响因子：
64.8
作者：
Heintzman, Nathaniel D.;Hon, Gary C.;Hawkins, R. David;Kheradpour, Pouya;Stark, Alexander;Harp, Lindsey F.;Ye, Zhen;Lee, Leonard K.;Stuart, Rhona K.;Ching, Christina W.;Ching, Keith A.;Antosiewicz-Bourget, Jessica E.;Liu, Hui;Zhang, Xinmin;Green, Roland D.;Lobanenkov, Victor V.;Stewart, Ron;Thomson, James A.;Crawford, Gregory E.;Kellis, Manolis;Ren, Bing
通讯作者：
Ren, Bing
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1073/pnas.0508647102
发表时间：
2005-11-29
影响因子：
11.1
作者：
Hwang, D;Rust, AG;Bolouri, H
通讯作者：
Bolouri, H
DOI：
10.3324/haematol.2011.060251
发表时间：
2012-09-01
期刊：
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
影响因子：
--
作者：
Bereshchenko, Oxana;Mancini, Elena;Nerlov, Claus
通讯作者：
Nerlov, Claus