Efficacy of the new β-D-glucan measurement kit for diagnosing invasive fungal infections, as compared with that of four conventional kits.
Efficacy of the new β-D-glucan measurement kit for diagnosing invasive fungal infections, as compared with that of four conventional kits.
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DOI：
10.1371/journal.pone.0255172
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Kikuchi T
中科院分区：
文献类型：
--
作者：
Bamba Y;Nagano K;Moro H;Ogata H;Hakamata M;Shibata S;Koizumi T;Aoki N;Ohshima Y;Watanabe S;Nakamura T;Kobayashi S;Hoshiyama Y;Koya T;Takada T;Kikuchi T
目前可用的每种（1→3）-β-D-葡聚糖（BDG）测量试剂盒均遵循不同的测量方法和临界值。侵袭性真菌感染（IFIs）的诊断性能的比较是可取的。此外，在开发新的测量工具包时，生态考虑变得越来越重要。使用以下现有试剂盒测量临床样本中的血浆BDG水平：Fungitec G test MKII、Fungitec G test ES、Fungitell、β-葡聚糖测试和子和新开发的和子试剂盒（Wako-Eu）。Wako-Eu使用符合欧洲化学品注册、评估、授权和限制法规的预处理解决方案。研究并比较了使用每种试剂盒获得的样品值。在165例患者中，12例有IFIs，包括肺孢子虫肺炎、曲霉病和念珠菌病。使用试剂盒获得的BDG值彼此中度相关。评价病例的临床诊断表明，至少有一种试剂盒诊断了21例假阳性。Fungitell试剂盒的灵敏度较低，而其他四种试剂盒的灵敏度均在90%以上。所有试剂盒的特异性均在90%以上。就阳性预测值而言，由于假阳性结果较少，和子法和Wako-Eu法优于其他方法，具有上级优势。新开发的Wako-Eu方法考虑了生态问题，其诊断性能与其前身相当。为了提高IFIs的诊断准确性，有必要仔细解释结果，适当考虑每个测量试剂盒的特性。
Each of the currently available (1→3)-β-D-glucan (BDG) measurement kits follows a different measurement method and cut-off value. Comparisons of diagnostic performance for invasive fungal infections (IFIs) are desirable. Additionally, ecological considerations are becoming increasingly important in the development of new measurement kits. The plasma BDG levels in clinical samples were measured using the following currently available kits: the Fungitec G test MKII, the Fungitec G test ES, Fungitell, the β-Glucan test Wako, and the newly developed Wako kit (Wako-Eu). Wako-Eu uses a pre-treatment solution that conforms to European regulations for the registration, evaluation, authorisation, and restriction of chemicals. The values obtained for the samples using each kit were studied and compared. Of the 165 patients evaluated, 12 had IFIs, including pneumocystis pneumonia, aspergillosis, and candidiasis. BDG values obtained using the kits were moderately correlated with each other. Clinical diagnoses of the evaluated cases indicated that 21 false positives were diagnosed by at least one kit. The sensitivity of the Fungitell kit was relatively low, but those of the other four were over 90%. The specificity was above 90% for all kits. For positive predictive value, the Wako and the Wako-Eu methods were superior to the others owing to fewer false positive results. The newly developed Wako-Eu method, which considers ecological concerns, shows diagnostic performance equivalent to that of its predecessor. To improve the diagnostic accuracy of IFIs, it is necessary to interpret the results carefully, giving due consideration to the characteristics of each measurement kit.
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影响因子：
3.7
作者：
Hou TY;Wang SH;Liang SX;Jiang WX;Luo DD;Huang DH
通讯作者：
Huang DH
影响因子：
168.9
作者：
OBAYASHI, T;YOSHIDA, M;KAWAI, T
通讯作者：
KAWAI, T
影响因子：
3.5
作者：
Tarrand, JJ;Lichterfeld, M;Kontoyiannis, DP
通讯作者：
Kontoyiannis, DP
影响因子：
9.4
作者：
Friedrich, Ricarda;Rappold, Elfriede;Held, Juergen
通讯作者：
Held, Juergen
影响因子：
11.8
作者：
Obayashi, Taminori;Negishi, Kumiko;Funata, Nobuaki
通讯作者：
Funata, Nobuaki