Stem blight of asparagus caused by <i>Phomopsis asparagi </i>in Myanmar and their phylogenetic grouping
Stem blight of asparagus caused by <i>Phomopsis asparagi </i>in Myanmar and their phylogenetic grouping
复制标题
缅甸拟茎点霉引起的芦笋茎枯病及其系统发育群
DOI：
10.11189/bita.42.23
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Masaru Matsumoto
中科院分区：
文献类型：
--
作者：
Myo Zaw;Seint San Aye;Le Dihn Thao;Masaru Matsumoto
2016年，缅甸中部地区Pyinmana附近的芦笋田发生茎枯病症状，并进行了样品采集。对芦笋病原真菌进行了分离，并对代表菌株进行了致病性鉴定。形态学特征表明，所有菌株产生的分生孢子含有α condinia马铃薯葡萄糖琼脂（PDA）培养基上。随机选取6个分离物进行rDNA-ITS区系统发育分析，结果表明，所有分离物与芦笋茎点霉（Phomopsis asparagi，GenBank登录号：KJ 801806和KJ 801804）同属一个分支。采用8个寡核苷酸引物对20个缅甸分离物和7个日本分离物进行随机扩增多态性DNA（RAPD）分析，用BOX和ERIC引物进行重复元件聚合酶链反应（rep-PCR）分析，比较其遗传多样性。在两个试验中，所有分离物的多态性带型相似，缅甸和日本分离物之间几乎没有差异。这是首次对缅甸芦笋上的芦笋疫霉进行鉴定。
In 2016, stem blight disease symptoms were occurred in asparagus fields near Pyinmana, central area of Myanmar, and samples collection was conducted. The causal fungal pathogen was isolated and the representative isolates were confirmed their pathogenicity on garden asparagus (Asparagus officinalis). The morphological characteristics indicated that all isolates produced conidiomata containing alpha condinia on potato dextrose agar (PDA) media. Six randomly selected isolates were performed on phylogenetic analysis of rDNA-ITS regions, and all isolates were in a single clade with Phomopsis asparagi (GenBank accession: KJ801806 and KJ801804). The genetic diversity of 20 isolates from Myanmar and 7 isolates from Japan were also compared by using random amplified polymorphic DNA (RAPD) analysis with eight oligo primers and repetitive-element polymerase chain reaction (rep-PCR) method with BOX and ERIC primers. In both experiment, the polymorphic banding patterns of all isolates were similar and there was almost no diversity between Myanmar and Japan isolates. This is the first characterization of P. asparagi on Asparagus in Myanmar.
登录
查看更多内容
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
果树拟茎点霉分离物核糖体 DNA 内转录间隔区的分子系统发育分析及繁殖力比较
影响因子：
1.2
作者：
S. Kanematsu;N. Minaka;Takao Kobayashi;A. Kudō;Y. Ohtsu
通讯作者：
Y. Ohtsu
DOI：
10.17660/actahortic.2012.950.34
发表时间：
2012
期刊：
--
影响因子：
--
作者：
Junyu Yin;C. Chin;Jinsong Ye;Weixing Zhao;Guodong Li
通讯作者：
Guodong Li
日本核核拟茎点霉引起的葫芦黑根腐病及其病原菌的系统发育分析
DOI：
--
发表时间：
2006
期刊：
Journal of General Plant Pathology 72(4)
影响因子：
--
作者：
Shishido;M.;Yoshida;N.;Usami;T.;Shinozaki;T.;Kobayashi;M.;Takeuchi;T.
通讯作者：
T.
通过rep-PCR对水稻纹枯病病原体缅甸丝核菌分离株进行遗传鉴定
影响因子：
2.2
作者：
Seint;Matsumoto
通讯作者：
Matsumoto