NF-κB protein expression associates with (18)F-FDG PET tumor uptake in non-small cell lung cancer: a radiogenomics validation study to understand tumor metabolism.
NF-κB protein expression associates with (18)F-FDG PET tumor uptake in non-small cell lung cancer: a radiogenomics validation study to understand tumor metabolism.
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DOI：
10.1016/j.lungcan.2013.11.001
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发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
West R
中科院分区：
文献类型：
--
作者：
Nair VS;Gevaert O;Davidzon G;Plevritis SK;West R
We previously demonstrated that NF-κB may be associated with 18F-FDG PET uptake and patient prognosis using radiogenomics in patients with non-small cell lung cancer (NSCLC). To validate these results, we assessed NF-κB protein expression in an extended cohort of NSCLC patients. We examined NF-κBp65 by immunohistochemistry (IHC) using a Tissue Microarray. Staining intensity was assessed by qualitative ordinal scoring and compared to tumor FDG uptake (SUVmax and SUVmean), Lactate Dehydrogenase A (LDHA) expression (as a positive control) and outcome using ANOVA, Kaplan Meier (KM), and Cox-proportional hazards (CPH) analysis. 365 tumors from 355 patients with long-term follow-up were analyzed. The average age for patients was 67 ± 11 years, 46% were male and 67% were ever smokers. Stage I and II patients comprised 83% of the cohort and the majority had adenocarcinoma (73%). From 88 FDG PET scans available, average SUVmax and SUVmean were 8.3 ±6.6, and 3.7 ±2.4 respectively. Increasing NF-κBp65 expression, but not LDHA expression, was associated with higher SUVmax and SUVmean (p = 0.03, 0.02 respectively). Both NF-κBp65 and positive FDG uptake were significantly associated with more advanced stage, tumor histology and invasion. Higher NF-κBp65 expression was associated with death by KM analysis (p = 0.06) while LDHA was strongly associated with recurrence (p = 0.04). Increased levels of combined NF-κBp65 and LDHA expression were synergistic and associated with both recurrence (p = 0.04) and death (p = 0.03). NF-κB IHC was a modest biomarker of prognosis that associated with tumor glucose metabolism on FDG PET when compared to existing molecular correlates like LDHA, which was synergistic with NF-κB for outcome. These findings recapitulate radiogenomics profiles previously reported by our group and provide a methodology for studying tumor biology using computational approaches.
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影响因子：
11.2
作者：
Nair VS;Gevaert O;Davidzon G;Napel S;Graves EE;Hoang CD;Shrager JB;Quon A;Rubin DL;Plevritis SK
通讯作者：
Plevritis SK
影响因子：
11.2
作者：
Dang CV
通讯作者：
Dang CV
影响因子：
5.3
作者：
Marom, EM;Aloia, TA;Patz, EF
通讯作者：
Patz, EF
影响因子：
21.3
作者：
Kawauchi, Keiko;Araki, Keigo;Tanaka, Nobuyuki
通讯作者：
Tanaka, Nobuyuki
影响因子：
5
作者：
Beck, Andrew H.;Espinosa, Inigo;West, Robert B.
通讯作者：
West, Robert B.