Phase behaviour of disordered proteins underlying low density and high permeability of liquid organelles.
Phase behaviour of disordered proteins underlying low density and high permeability of liquid organelles.
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DOI：
10.1038/nchem.2803
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发表时间：
2017-11
期刊：
影响因子：
21.8
通讯作者：
Brangwynne CP
中科院分区：
文献类型：
--
作者：
Wei MT;Elbaum-Garfinkle S;Holehouse AS;Chen CC;Feric M;Arnold CB;Priestley RD;Pappu RV;Brangwynne CP
许多细胞内无膜细胞器通过内在无序蛋白质/区域（IDP/IDR）的相分离形成。这些包括线虫蛋白LAF-1，其在体外形成P-颗粒样液滴。然而，蛋白质无序在相分离和液滴内大分子组织中的作用仍然难以捉摸。在这里，我们利用一种新的技术，超快扫描荧光相关光谱，测量分子相互作用和完整的共存曲线（双节点），量化LAF-1液滴内的蛋白质浓度。LAF-1及其IDR的双结点显示出许多不寻常的特征，包括“高浓度”的双结点臂，对应于显着稀释液滴。我们发现，LAF-1和其他体外和细胞内液滴的特征在于约3-8 nm的有效网格尺寸，这决定了液滴特性影响分子扩散和渗透性的尺寸尺度。这些发现揭示了特定的IDP如何相分离以形成可渗透的低密度（半稀释）液体，其结构特征可能会强烈影响生物功能。
Many intracellular membraneless organelles form via phase separation of intrinsically disordered proteins/regions (IDPs/IDRs). These include the C elegans protein LAF-1, which forms P-granule-like droplets in vitro. However, the role of protein disorder in phase separation and the macromolecular organization within droplets remain elusive. Here, we utilize a novel technique, ultrafast-scanning fluorescence correlation spectroscopy, to measure the molecular interactions and full coexistence curves (binodals), which quantify the protein concentration within LAF-1 droplets. The binodals of LAF-1 and its IDR display a number of unusual features, including “high concentration” binodal arms that correspond to remarkably dilute droplets. We find that LAF-1 and other in vitro and intracellular droplets are characterized by an effective mesh size of ~3–8 nm, which determines the size scale at which droplet properties impact molecular diffusion and permeability. These findings reveal how specific IDPs can phase separate to form permeable, low-density (semidilute) liquids, whose structural features are likely to strongly impact biological function.
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影响因子：
3.8
作者：
Duocastella, Marti;Vicidomini, Giuseppe;Diaspro, Alberto
通讯作者：
Diaspro, Alberto
影响因子：
8.6
作者：
Lee, Chiu Fan;Brangwynne, Clifford P.;Juelicher, Frank
通讯作者：
Juelicher, Frank
影响因子：
21.8
作者：
Aumiller, William M., Jr.;Keating, Christine D.
通讯作者：
Keating, Christine D.
DOI：
10.1073/pnas.1509317112
发表时间：
2015-09-22
影响因子：
11.1
作者：
Berry, Joel;Weber, Stephanie C.;Brangwynne, Clifford P.
通讯作者：
Brangwynne, Clifford P.
影响因子：
64.5
作者：
Feric M;Vaidya N;Harmon TS;Mitrea DM;Zhu L;Richardson TM;Kriwacki RW;Pappu RV;Brangwynne CP
通讯作者：
Brangwynne CP