Composite genome map and recombination parameters derived from three archetypal lineages of Toxoplasma gondii.
Composite genome map and recombination parameters derived from three archetypal lineages of Toxoplasma gondii.
复制标题
DOI：
10.1093/nar/gki604
复制
发表时间：
2005
影响因子：
14.9
通讯作者：
Sibley LD
中科院分区：
文献类型：
--
作者：
Khan A;Taylor S;Su C;Mackey AJ;Boyle J;Cole R;Glover D;Tang K;Paulsen IT;Berriman M;Boothroyd JC;Pfefferkorn ER;Dubey JP;Ajioka JW;Roos DS;Wootton JC;Sibley LD
刚地弓形虫是顶复合体门中一种非常成功的原生动物寄生虫，它含有许多动物和人类病原体。弓形虫适合于细胞、生化、分子和遗传研究，使其成为这一重要寄生虫群体的生物学模型。为了便于前向遗传分析，我们开发了一个高分辨率的弓形虫遗传连锁图谱。该遗传图谱用于组装来自10X霰弹枪全基因组序列的支架，从而确定了基因组中间隔约300 kb的14条染色体的标记。鉴定出14条染色体，总遗传大小为~ 592 cM，平均图谱单位为~ 104 kb/cM。对弓形虫遗传参数的分析显示，高频率的密切相邻、明显的双交叉事件可能代表基因转换。此外，我们在原型克隆谱系中发现了大面积的遗传同质性，这反映了自它们最近起源以来发生的相对较少的遗传远交事件。尽管这些不寻常的特征，连锁分析证明是有效的定位基因座决定几种耐药性。由此产生的基因组图谱为毒力和传播等复杂性状的分析以及比较群体遗传研究提供了一个框架。
Toxoplasma gondii is a highly successful protozoan parasite in the phylum Apicomplexa, which contains numerous animal and human pathogens. T.gondii is amenable to cellular, biochemical, molecular and genetic studies, making it a model for the biology of this important group of parasites. To facilitate forward genetic analysis, we have developed a high-resolution genetic linkage map for T.gondii. The genetic map was used to assemble the scaffolds from a 10X shotgun whole genome sequence, thus defining 14 chromosomes with markers spaced at ∼300 kb intervals across the genome. Fourteen chromosomes were identified comprising a total genetic size of ∼592 cM and an average map unit of ∼104 kb/cM. Analysis of the genetic parameters in T.gondii revealed a high frequency of closely adjacent, apparent double crossover events that may represent gene conversions. In addition, we detected large regions of genetic homogeneity among the archetypal clonal lineages, reflecting the relatively few genetic outbreeding events that have occurred since their recent origin. Despite these unusual features, linkage analysis proved to be effective in mapping the loci determining several drug resistances. The resulting genome map provides a framework for analysis of complex traits such as virulence and transmission, and for comparative population genetic studies.
登录
查看更多内容
影响因子：
14.9
作者：
Li, L;Crabtree, J;Roos, DS
通讯作者：
Roos, DS
影响因子：
2.5
作者：
Manly, KF;Cudmore, RH;Meer, JM
通讯作者：
Meer, JM
影响因子：
16
作者：
Fidock, DA;Nomura, T;Wellems, TE
通讯作者：
Wellems, TE
影响因子：
3.1
作者：
Manger, ID;Hehl, A;Boothroyd, JC
通讯作者：
Boothroyd, JC
影响因子：
3.6
作者：
Mu, JB;Ferdig, MT;Su, XZ
通讯作者：
Su, XZ