Molecular cloning and nucleotide sequence analysis of psbA from the dinoflagellates: Origin of the dinoflagellate plastid
Molecular cloning and nucleotide sequence analysis of psbA from the dinoflagellates: Origin of the dinoflagellate plastid
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甲藻 psbA 的分子克隆和核苷酸序列分析：甲藻质体的起源
DOI：
10.1046/j.1440-1835.1999.00163.x
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发表时间：
1999
影响因子：
1.5
通讯作者：
A. Uchida
中科院分区：
文献类型：
--
作者：
K. Takishita;A. Uchida
psbA 的克隆和测序，该基因编码光系统 II 的 D1 蛋白，来自六种含有多甲素型质体的甲藻 [Prorocentrum micans Ehrenberg、Amphidinium carterae Hulburt、Heterocapsa triquetra Stein、Lingulodinium polyedra (Dodge) Stein、Alexandrium tamarense (Lebour) Balech 和 Alexandrium catenella (Whedon et)据报道，Kofoid）Balech]。使用聚合酶链式反应技术，通过 CsCl-Hoechst 33258 梯度超速离心从链状链霉菌总 DNA 中分离出卫星 DNA 带，检测到 psbA 基因。这一发现表明，在甲藻中，psbA 是在质体基因组中编码的。从甲藻中推导的 D1 氨基酸序列没有揭示出典型的“C 末端延伸”，这应该通过 D1 前体的蛋白水解切割来去除。基于D1推导的氨基酸序列的分子系统发育分析表明，这6种甲藻是单系的，并且还表明甲藻与红藻类、隐藻类和异形藻类聚在一起。这些结果支持甲藻中的多甲素型质体起源于被吞噬的红藻的假设。
Cloning and sequencing of psbA, the gene encoding D1 protein of photosystem II, from six species of dinoflagellates harboring a peridinin type plastid [Prorocentrum micans Ehrenberg, Amphidinium carterae Hulburt, Heterocapsa triquetra Stein, Lingulodinium polyedra (Dodge) Stein, Alexandrium tamarense (Lebour) Balech and Alexandrium catenella (Whedon et Kofoid) Balech] is reported. Using the polymerase chain reaction technique, the psbA gene was detected in a satellite DNA band isolated from total DNA of A. catenella by CsCl‐Hoechst 33258 gradient ultracentrifugation. This finding suggests that in dinoflagellates psbA is encoded in the plastid genome. The deduced amino acid sequences of D1 from the dinoflagellates did not reveal a typical ‘C‐terminus extension’, which should be removed by proteolytic cleavage from the D1 precursor. Molecular phylogenetic analysis based on the deduced amino acid sequences of D1 revealed that the six species of dinoflagellates are monophyletic and also showed that dinoflagellates cluster with rhodophytes, a cryptophyte and heterokonts. These results support the hypothesis that the peridinin type plastid in dinoflagellates originated from an engulfed red alga.
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR
影响因子：
56.9
作者：
MORSE, D;SALOIS, P;HASTINGS, JW
通讯作者：
HASTINGS, JW
影响因子：
1.5
作者：
GAJADHAR, AA;MARQUARDT, WC;SOGIN, ML
通讯作者：
SOGIN, ML