Cell wall fucosylation in Arabidopsis influences control of leaf water loss and alters stomatal development and mechanical properties.
Cell wall fucosylation in Arabidopsis influences control of leaf water loss and alters stomatal development and mechanical properties.
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DOI：
10.1093/jxb/erad039
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发表时间：
2023-04-18
影响因子：
6.9
通讯作者：
中科院分区：
文献类型：
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作者：
拟南芥对冷冻敏感8（sfr8）突变体表现出细胞壁（CW）岩藻糖水平降低和抗冻性受损。为了检验CW岩藻糖基化是否也影响对干燥的响应，我们测试了在sfr8和等位基因突变体mur1 - 1中叶片切除的效果。叶片水分损失显着高于野生型在这些，但不是其他，岩藻糖基化突变体。我们推测，减少岩藻糖基化保卫细胞（GC）壁可能会限制气孔关闭，通过改变机械性能。多频原子力显微镜（AFM）的测量结果显示，减少弹性模量（E），代表减少刚度，在SFR8 GC的墙壁。然而，有趣的是，我们发现了一种补偿机制，由此伴随的储能模量（E_（max））的降低在sfr 8中维持野生型粘弹性时间响应（tau）。sfr8完整叶盘中的气孔对关闭刺激脱落酸的反应正常，这表明时间反应可能比刚度更与关闭特性有关。sfr8气孔复合体比野生型的气孔复合体大，GCs缺乏充分发育的角质壁架，这两个潜在的贡献者在sfr8更大的叶片水分损失。我们目前的数据表明，岩藻糖基化依赖的CW果胶结构域鼠李糖半乳糖醛酸-II的二聚化可能是必不可少的正常表皮壁架的发展和叶片保水。我们表明，岩藻糖依赖的果胶交联是所需的正常气孔发育和控制叶片失水。岩藻糖基化缺陷突变体的保卫细胞壁硬度降低并不影响气孔关闭。
The Arabidopsis sensitive-to-freezing8 (sfr8) mutant exhibits reduced cell wall (CW) fucose levels and compromised freezing tolerance. To examine whether CW fucosylation also affects the response to desiccation, we tested the effect of leaf excision in sfr8 and the allelic mutant mur1-1. Leaf water loss was strikingly higher than in the wild type in these, but not other, fucosylation mutants. We hypothesized that reduced fucosylation in guard cell (GC) walls might limit stomatal closure through altering mechanical properties. Multifrequency atomic force microscopy (AFM) measurements revealed a reduced elastic modulus (Eʹ), representing reduced stiffness, in sfr8 GC walls. Interestingly, however, we discovered a compensatory mechanism whereby a concomitant reduction in the storage modulus (Eʹʹ) maintained a wild-type viscoelastic time response (tau) in sfr8. Stomata in intact leaf discs of sfr8 responded normally to a closure stimulus, abscisic acid, suggesting that the time response may relate more to closure properties than stiffness does. sfr8 stomatal pore complexes were larger than those of the wild type, and GCs lacked a fully developed cuticular ledge, both potential contributors to the greater leaf water loss in sfr8. We present data that indicate that fucosylation-dependent dimerization of the CW pectic domain rhamnogalacturonan-II may be essential for normal cuticular ledge development and leaf water retention. We show that fucose-dependent pectin cross-linking is required for normal stomatal development and control of leaf water loss. Reduced guard cell wall stiffness in a fucosylation-defective mutant did not influence stomatal closure.
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DOI：
10.1073/pnas.0706984104
发表时间：
2007-11-13
影响因子：
11.1
作者：
Li, Yonghua;Beisson, Fred;Ohlrogge, John
通讯作者：
Ohlrogge, John
影响因子：
5.6
作者：
Lee M;Dominguez-Ferreras A;Kaliyadasa E;Huang WJ;Antony E;Stevenson T;Lehmann S;Schäfer P;Knight MR;Ntoukakis V;Knight H
通讯作者：
Knight H
影响因子：
7.4
作者：
Kobayashi, M;Matoh, T;Azuma, J
通讯作者：
Azuma, J
DOI：
10.1073/pnas.94.5.2085
发表时间：
1997-03-04
影响因子：
11.1
作者：
Bonin, CP;Potter, I;Reiter, WD
通讯作者：
Reiter, WD
影响因子：
9.2
作者：
Amsbury, Sam;Hunt, Lee;Elhaddad, Nagat;Baillie, Alice;Lundgren, Marjorie;Verhertbruggen, Yves;Scheller, Henrik V.;Knox, J. Paul;Fleming, Andrew J.;Gray, Julie E.
通讯作者：
Gray, Julie E.