Mediator Subunits MED16, MED14, and MED2 Are Required for Activation of ABRE-Dependent Transcription in Arabidopsis.
Mediator Subunits MED16, MED14, and MED2 Are Required for Activation of ABRE-Dependent Transcription in Arabidopsis.
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DOI：
10.3389/fpls.2021.649720
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发表时间：
2021
影响因子：
5.6
通讯作者：
Knight H
中科院分区：
文献类型：
--
作者：
Lee M;Dominguez-Ferreras A;Kaliyadasa E;Huang WJ;Antony E;Stevenson T;Lehmann S;Schäfer P;Knight MR;Ntoukakis V;Knight H
介体复合物控制大多数真核基因的转录，其具有响应于各种扰动控制特定基因调节子所需的单个亚基。在这项研究中，我们揭示了植物介体亚基MED16，MED14和MED2在调节转录响应植物激素脱落酸（阿坝）的作用，我们确定哪些顺式元件是在他们的控制下。使用合成的启动子报告，我们建立了一个有效的系统，用于测试亚基和特定的顺式作用基序在原生质体之间的关系。我们的研究结果表明，MED16，MED14和MED2所需的阿坝的启动子包含ABRE（ABA响应元件）和DRE（干旱响应元件）的完全转录激活。使用合成的启动子基序多联体，我们表明，ABA响应激活的ABRE，但不是DRE基序依赖于这三个介体亚基。此外，这三个亚基是控制叶片失水所必需的，但在ABA依赖的生长抑制中不起作用，突出了它们功能的特异性。我们的研究结果确定了三个中介子亚基的新角色，提供了一个直接的演示，他们的功能，并强调，我们的实验方法可以用来确定植物转录调节子亚基的功能。
The Mediator complex controls transcription of most eukaryotic genes with individual subunits required for the control of particular gene regulons in response to various perturbations. In this study, we reveal the roles of the plant Mediator subunits MED16, MED14, and MED2 in regulating transcription in response to the phytohormone abscisic acid (ABA) and we determine which cis elements are under their control. Using synthetic promoter reporters we established an effective system for testing relationships between subunits and specific cis-acting motifs in protoplasts. Our results demonstrate that MED16, MED14, and MED2 are required for the full transcriptional activation by ABA of promoters containing both the ABRE (ABA-responsive element) and DRE (drought-responsive element). Using synthetic promoter motif concatamers, we showed that ABA-responsive activation of the ABRE but not the DRE motif was dependent on these three Mediator subunits. Furthermore, the three subunits were required for the control of water loss from leaves but played no role in ABA-dependent growth inhibition, highlighting specificity in their functions. Our results identify new roles for three Mediator subunits, provide a direct demonstration of their function and highlight that our experimental approach can be utilized to identify the function of subunits of plant transcriptional regulators.
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影响因子：
14.9
作者：
Gibson DG
通讯作者：
Gibson DG
DOI：
10.1534/g3.118.200573
发表时间：
2018-08-30
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Dolan WL;Chapple C
通讯作者：
Chapple C
影响因子：
4.6
作者：
Crawford, Tim;Karamat, Fazeelat;Bjorklund, Stefan
通讯作者：
Bjorklund, Stefan
影响因子：
16
作者：
Backstrom, Stefan;Elfving, Nils;Bjorklund, Stefan
通讯作者：
Bjorklund, Stefan
影响因子：
16.8
作者：
Cevher MA;Shi Y;Li D;Chait BT;Malik S;Roeder RG
通讯作者：
Roeder RG