MiRNA-30a inhibits AECs-II apoptosis by blocking mitochondrial fission dependent on Drp-1.
MiRNA-30a inhibits AECs-II apoptosis by blocking mitochondrial fission dependent on Drp-1.
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DOI：
10.1111/jcmm.12420
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发表时间：
2014-12
影响因子：
5.3
通讯作者：
Lv C
中科院分区：
文献类型：
--
作者：
Mao C;Zhang J;Lin S;Jing L;Xiang J;Wang M;Wang B;Xu P;Liu W;Song X;Lv C
Apoptosis of type II alveolar epithelial cells (AECs-II) is a key determinant of initiation and progression of lung fibrosis. However, the mechanism of miR-30a participation in the regulation of AECs-II apoptosis is ambiguous. In this study, we investigated whether miR-30a could block AECs-II apoptosis by repressing mitochondrial fission dependent on dynamin-related protein-1 (Drp-1). The levels of miR-30a in vivo and in vitro were determined through quantitative real-time PCR (qRT-PCR). The inhibition of miR-30a in AECs-II apoptosis, mitochondrial fission and its dependence on Drp-1, and Drp-1 expression and translocation were detected using miR-30a mimic, inhibitor-transfection method (gain- and loss-of-function), or Drp-1 siRNA technology. Results showed that miR-30a decreased in lung fibrosis. Gain- and loss-of-function studies revealed that the up-regulation of miR-30a could decrease AECs-II apoptosis, inhibit mitochondrial fission, and reduce Drp-1 expression and translocation. MiR-30a mimic/inhibitor and Drp-1 siRNA co-transfection showed that miR-30a could inhibit the mitochondrial fission dependent on Drp-1. This study demonstrated that miR-30a inhibited AECs-II apoptosis by repressing the mitochondrial fission dependent on Drp-1, and could function as a novel therapeutic target for lung fibrosis.
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影响因子：
7.7
作者：
Bhatt MP;Lim YC;Kim YM;Ha KS
通讯作者：
Ha KS
影响因子：
3.5
作者：
Lu, Tien Hui;Chen, Chun Hung;Chen, Ya Wen
通讯作者：
Chen, Ya Wen
影响因子：
--
作者：
Otera H;Mihara K
通讯作者：
Mihara K
DOI：
10.1073/pnas.1321114111
发表时间：
2014-04-29
影响因子：
11.1
作者：
Kim, Seong-Jun;Syed, Gulam H.;Siddiqui, Aleem
通讯作者：
Siddiqui, Aleem
DOI：
10.1152/ajplung.00099.2011
发表时间：
2012-03-01
影响因子：
4.9
作者：
Bhandary, Yashodhar P.;Shetty, Shwetha K.;Shetty, Sreerama
通讯作者：
Shetty, Sreerama