Association between VDAC1 mRNA expression and intracellular ATP levels of cultured L-02 hepatocytes during hexavalent chromium toxicity
Association between VDAC1 mRNA expression and intracellular ATP levels of cultured L-02 hepatocytes during hexavalent chromium toxicity
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六价铬中毒期间培养的 L-02 肝细胞 VDAC1 mRNA 表达与细胞内 ATP 水平之间的关联
DOI：
10.5897/ajb11.3120
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发表时间：
2011-01
影响因子：
--
通讯作者：
Zhong Cai-Gao
中科院分区：
文献类型：
--
作者：
Yang Yuan;Li Peng;Hu Gong-Hua;Zhou Yu;Dai Lu;Zhong Cai-Gao
外源性物质诱导凋亡性细胞死亡的一种方式是改变线粒体膜中细胞内电压依赖性阴离子通道（VDAC 1）的选择性渗透性。在这项研究中，我们探讨了六价铬化合物[Cr（VI）]处理过程中VDAC 1 mRNA表达和线粒体功能之间的关联。培养的L-02肝细胞分别用2、4、8、16或32 μmol/L Cr（VI）处理12、24或36 h。VDAC 1 mRNA的表达通过定性逆转录聚合酶链反应（RT-PCR）测定，而细胞内三磷酸腺苷（ATP）水平通过ATP特异性生物发光测定测定。在此范围内的Cr（VI）浓度，平均VDAC 1 mRNA表达下降84%后，12小时的治疗和40%后，24小时的治疗，但它增加了2.33倍，在36小时相比，与对照培养物。用Cr（VI）处理破坏细胞代谢，如ATP水平的变化所证明的。Cr（VI）处理引起ATP水平在12 h升高，24 h降低，36 h再次升高，导致倾斜的V形曲线。相关分析显示，在Cr（VI）处理过程中，VDAC 1 mRNA表达与细胞内ATP水平呈中度负相关（r =-0.557，P < 0.05）。在32 μmol/L Cr（VI）组中，这种负相关性更为明显。基于这些结果，重金属毒性可能会诱导VDAC 1 mRNA的过度表达，从而导致ATP水平下降。关键词：重金属毒性，VDAC 1 mRNA表达，ATP，关系。
One way in which xenobiotics induce apoptotic cell death is to alter the selective permeability of the intracellular voltage-dependent anion channel (VDAC1) in the mitochondrial membrane. In this study, we explored the association between VDAC1 mRNA expression and mitochondrial function during hexavalent chromium compounds [Cr(VI)] treatment. Cultured L-02 hepatocytes were treated with 2, 4, 8, 16, or 32 μmol/L Cr(VI) for 12, 24, or 36 h. Expression of VDAC1 mRNA was measured by qualitative reverse transcription polymerase chain reaction (RT-PCR), whereas intracellular adenosine triphosphate (ATP) levels were determined by an ATP-specific bioluminescence assay. Over this range of Cr(VI) concentrations, average VDAC1 mRNA expression decreased by 84% after 12 h treatment and by 40% after 24 h of treatment, but it increased 2.33-fold at 36 h when compared with the control cultures. Treatment with Cr(VI) disrupted cellular metabolism as evidenced by changes in ATP levels. Cr(VI) treatment caused ATP levels to increase at 12 h, decrease at 24 h, and increase again at 36 h, resulting in a slant V-shaped curve. Correlation analysis revealed a moderate negative relationship between VDAC1 mRNA expression and intracellular ATP levels during Cr(VI) treatment (r = -0.557, P < 0.05). The negative association seemed to be more obvious in 32 μmol/L Cr(VI) group. Based on these results, heavy metal toxicity may induce over-expression of VDAC1 mRNA, which causes a decrease in ATP levels. Key words : Heavy metal toxicity, VDAC1 mRNA expression, ATP, relationship.
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DOI：
--
发表时间：
2006-07
期刊：
Wei sheng yan jiu = Journal of hygiene research
影响因子：
--
作者：
Jing-wei Xiao;Cai-gao Zhong;Bin Li
通讯作者：
Jing-wei Xiao;Cai-gao Zhong;Bin Li
影响因子：
3.8
作者：
Son YO;Hitron JA;Wang X;Chang Q;Pan J;Zhang Z;Liu J;Wang S;Lee JC;Shi X
通讯作者：
Shi X
影响因子：
3
作者：
Gincel, D;Silberberg, SD;Shoshan-Barmatz, V
通讯作者：
Shoshan-Barmatz, V
DOI：
--
发表时间：
1997
期刊：
--
影响因子：
--
作者：
E. Forte;J. Blachly-Dyson;W. J. Song;M. Wolfgang;M. Colombini
通讯作者：
E. Forte;J. Blachly-Dyson;W. J. Song;M. Wolfgang;M. Colombini
影响因子：
4.6
作者：
E. Blachly‐Dyson;M. Forte
通讯作者：
E. Blachly‐Dyson;M. Forte