Detection and characterization of [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin binding to Ah receptor in a rainbow trout hepatoma cell line.
Detection and characterization of [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin binding to Ah receptor in a rainbow trout hepatoma cell line.
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虹鳟鱼肝癌细胞系中 [3H]2,3,7,8-四氯二苯并-对-二恶英与 Ah 受体结合的检测和表征。
DOI：
10.1016/0041-008x(90)90105-4
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发表时间：
1990
影响因子：
3.8
通讯作者：
A. Okey
中科院分区：
文献类型：
--
作者：
Angela Lorenzen;A. Okey
在虹鳟肝癌细胞系RTH-149的胞浆和核提取物中鉴定并表征了Ah受体。细胞溶质受体可检测到卤代（[3 H] 2，3，7，8-四氯二苯并-p-二恶英（TCDD））和非卤代（[3 H]3-甲基胆蒽和[3 H]苯并[a]芘）芳烃，并在蔗糖梯度上的速度沉降后，在100 - 9 S沉降。Scatchard或Woolf曲线分析表明，胞浆Ah受体的表观结合亲和力（kd）均小于1 nm。同样的分析表明，RTH-149细胞中Ah受体的浓度为1020 fmol/mg胞质蛋白或每个细胞约4400个受体位点。因此，这种鳟鱼肝癌细胞系具有低浓度的高亲和力结合位点相比，Ah受体浓度从啮齿动物组织获得的胞质溶胶。孵育的全细胞与放射性配体[3 H]TCDD导致转化的胞质Ah受体的核结合的形式，可以检测到作为一个特定的标记峰沉降在蔗糖梯度在1006 S。RTH-149细胞在培养物中暴露于TCDD或苯并[a]蒽24小时后，芳烃羟化酶被诱导。这些数据表明，在来自非哺乳动物物种的细胞系中的Ah受体的存在，并提供了一个额外的步骤，了解鱼类响应特定的水生污染物的机制。
Ah receptor was identified and characterized in cytosol and nuclear extracts from the rainbow trout hepatoma cell line RTH-149. The cytosolic receptor was detectable with both halogenated ([3H]2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)) and non-halogenated ([3H]3-methylcholanthrene and [3H]benzo[a]pyrene) aromatic hydrocarbons and sedimented at ∼9 S after velocity sedimentation on sucrose gradients. The apparent binding affinity (kd) of cytosolic Ah receptor was always less than 1 nm as derived from Scatchard or Woolf plot analyses. The same analyses indicated a concentration of Ah receptor in the RTH-149 cells of ∼20 fmol/mg cytosolic protein or approximately 4400 receptor sites per cell. Thus, this trout hepatoma cell line has a low concentration of high-affinity binding sites in comparison to Ah receptor concentrations in cytosol obtained from rodent tissues. Incubation of whole cells with the radioligand [3H]TCDD resulted in transformation of the cytosolic Ah receptor to a nuclear binding form which could be detected as a specifically labeled peak sedimenting at ∼6 S on sucrose gradients. Aryl hydrocarbon hydroxylase was induced after exposure of RTH-149 cells to TCDD or benz[a]anthracene for 24 hr in culture. These data demonstrate the existence of the Ah receptor in a cell line derived from a nonmammalian species and provide an additional step toward understanding the mechanisms by which fish respond to specific aquatic contaminants.
用 2,3,7,8-四氯二苯并-p-二恶英处理的虹鳟鱼 (Salmo gairdneri) 的形态损伤和急性毒性。
DOI：
10.1080/15287398809531119
发表时间：
1988
期刊：
Journal of toxicology and environmental health
影响因子：
--
作者：
Spitsbergen,JM;Kleeman,JM;Peterson,RE
通讯作者：
Peterson,RE
生物转化和诱导：对鱼类环境异生物质的毒性、生物累积和监测的影响。
影响因子：
10.4
作者：
Kleinow,KM;Melancon,MJ;Lech,JJ
通讯作者：
Lech,JJ