Probe Electrospray Ionization Mass Spectrometry with Discontinuous Atmospheric Pressure Interface
Probe Electrospray Ionization Mass Spectrometry with Discontinuous Atmospheric Pressure Interface
复制标题
具有不连续大气压接口的探针电喷雾电离质谱法
DOI：
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发表时间：
2015
影响因子：
1.3
通讯作者：
K. Hiraoka
中科院分区：
文献类型：
--
作者：
D. Usmanov;Subhrakanti Saha;L. Chen;S. Ninomiya;M. K. Mandal;K. Hiraoka
使用外径为0.2 mm的钛丝进行探针电喷雾电离（PESI），并使用夹管阀通过不连续的大气压接口将产生的离子引入质谱仪。延时PESI质谱通过逐渐增加夹管阀打开相对于施加高电压时每个电喷雾事件开始的延迟时间来获得。夹管阀的打开时间为20 ms。时间分辨PESI质谱显示，在加入和不加入10 mM CH 3COONH 4的情况下，10 mM NaCl、10−5 M短杆菌肽S和胰岛素在H2O/CH 3OH/CH 3COOH（65/35/1）中的浓度存在显著差异。这归因于在金属探针和溶液之间的界面处发生的电化学反应引起的附接到针的液体的pH变化。NaCl团簇离子出现后，耗尽的分析物。对于10−5 M细胞色素c、胰岛素和短杆菌肽S在H2O/CH 3OH/CH 3COOH（65/35/1）中的混合溶液，观察到分析物离子按细胞色素c →胰岛素→短杆菌肽S的顺序依次出现。本技术应用于三种麻醉品样品，甲基苯丙胺，吗啡和可待因。对于夹管阀打开时间为200 ms的单次取样，这些化合物的检测限为10 ppb（H2O/CH 3OH（1/1））。
Probe electrospray ionization (PESI) using a 0.2 mm outside diameter titanium wire was performed and the generated ions were introduced into the mass spectrometer via a discontinuous atmospheric pressure interface using a pinch valve. Time-lapse PESI mass spectra were acquired by gradually increasing delay time for the pinch valve opening with respect to the start of each electrospray event when a high voltage was applied. The opening time of the pinch valve was 20 ms. Time-resolved PESI mass spectra showed marked differences for 10 mM NaCl, 10−5 M gramicidin S and insulin in H2O/CH3OH/CH3COOH (65/35/1) with and without the addition of 10 mM CH3COONH4. This was ascribed to the pH change of the liquid attached to the needle caused by electrochemical reactions taking place at the interface between the metal probe and the solution. NaCl cluster ions appeared only after the depletion of analytes. For the mixed solution of 10−5 M cytochrome c, insulin, and gramicidin S in H2O/CH3OH/CH3COOH (65/35/1), a sequential appearance of analyte ions in the order of cytochrome c → insulin → gramicidin S was observed. The present technique was applied to three narcotic samples, methamphetamine, morphine, and codeine. Limits of detection for these compounds were 10 ppb in H2O/CH3OH (1/1) for the single sampling with a pinch valve opening time of 200 ms.
触摸喷雾质谱法，用于对复杂样品的原位分析。
DOI：
10.1039/c4an00548a
发表时间：
2014-06-07
期刊：
The Analyst
影响因子：
--
作者：
Kerian KS;Jarmusch AK;Cooks RG
通讯作者：
Cooks RG