Mesoporous silica chips for selective enrichment and stabilization of low molecular weight proteome.
Mesoporous silica chips for selective enrichment and stabilization of low molecular weight proteome.
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DOI：
10.1002/pmic.200900346
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发表时间：
2010-02
期刊：
影响因子：
3.4
通讯作者：
Ferrari, Mauro
中科院分区：
文献类型：
--
作者：
Bouamrani, Ali;Hu, Ye;Tasciotti, Ennio;Li, Li;Chiappini, Ciro;Liu, Xuewu;Ferrari, Mauro
关键词：
介孔二氧化硅的先进特性已在化学传感、过滤、催化、药物输送和选择性生物分子摄取等应用中得到证明。这些特性取决于材料的结构、物理和化学特性，而这些特性又由蒸发诱导自组装的工艺参数决定。在这项研究中，我们引入了一种组合方法，用于去除高分子量蛋白质以及特定分离和富集低分子量物种。该方法基于介孔二氧化硅芯片，能够分馏、选择性收获复杂人体生物体液中存在的肽和蛋白质，并防止其被酶促降解。我们使用肽和蛋白质标准库描述了各种介孔芯片的收获特性及其对血清肽组回收的选择性。使用基质辅助激光解吸/电离飞行时间质谱法，我们建立了介孔二氧化硅表面的收获特异性和物理化学性质之间的相关性。这种具有精细受控特性的介孔材料的引入将为蛋白质组学应用提供强大的平台，为低分子量生物标志物的发现提供快速有效的方法。
The advanced properties of mesoporous silica have been demonstrated in applications which include chemical sensing, filtration, catalysis, drug-delivery and selective biomolecular uptake. These properties depend on the architectural, physical and chemical properties of the material, which in turn are determined by the processing parameters in evaporation-induced self-assembly. In this study, we introduce a combinatorial approach for the removal of the high molecular weight proteins and for the specific isolation and enrichment of low molecular weight species. This approach is based on Mesoporous Silica Chips able to fractionate, selectively harvest and protect from enzymatic degradation, peptides and proteins present in complex human biological fluids. We present the characterization of the harvesting properties of a wide range of mesoporous chips using a library of peptides and proteins standard and their selectivity on the recovery of serum peptidome. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, we established the correlation between the harvesting specificity and the physico-chemical properties of mesoporous silica surfaces. The introduction of this mesoporous material with fine controlled properties will provide a powerful platform for proteomics application offering a rapid and efficient methodology for low molecular weight biomarker discovery.
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