TGF-beta mediated FGF10 signaling in cranial neural crest cells controls development of myogenic progenitor cells through tissue-tissue interactions during tongue morphogenesis.
TGF-beta mediated FGF10 signaling in cranial neural crest cells controls development of myogenic progenitor cells through tissue-tissue interactions during tongue morphogenesis.
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DOI：
10.1016/j.ydbio.2010.02.030
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发表时间：
2010-05-01
影响因子：
2.7
通讯作者：
Chai Y
中科院分区：
文献类型：
--
作者：
Hosokawa R;Oka K;Yamaza T;Iwata J;Urata M;Xu X;Bringas P Jr;Nonaka K;Chai Y
骨骼肌由肌源性和成纤维细胞两种细胞谱系组成。中胚层来源的肌祖细胞形成肌肉细胞，而成纤维细胞则形成骨骼肌的支持性结缔组织，如肌腱和肌束膜。目前尚不清楚肌源性和成纤维细胞间的相互作用如何影响细胞命运的决定和骨骼肌的组织。在本研究中，我们研究了细胞-细胞相互作用在调节骨骼肌发育中的功能意义。我们的研究表明，小鼠的舌骨骼肌成纤维细胞是由脑神经脊细胞分化而来的。Cnt细胞(Wnt1-Cre；Tgfbr2Flox/Flox)中TGFBR2的缺失导致小舌症，硬化轴和Fgf10表达减少，肌源性细胞增殖减少，细胞数量减少，舌肌组织紊乱。此外，转化生长因子-β-2微球还可诱导舌外植体培养中硬化轴的表达。FGF10的加入挽救了Wnt1-Cre；Tgfbr2Flox/Flox小鼠的肌细胞数量。因此，转化生长因子-β诱导的成纤维细胞生长因子10信号在调节舌骨骼肌发育过程中的组织-组织相互作用中起着关键作用。
Skeletal muscles are formed from two cell lineages, myogenic and fibroblastic. Mesoderm-derived myogenic progenitors form muscle cells whereas fibroblastic cells give rise to the supportive connective tissue of skeletal muscles, such as the tendons and perimysium. It remains unknown how myogenic and fibroblastic cell-cell interactions affect cell fate determination and the organization of skeletal muscle. In the present study, we investigated the functional significance of cell-cell interactions in regulating skeletal muscle development. Our study shows that cranial neural crest (CNC) cells give rise to the fibroblastic cells of the tongue skeletal muscle in mice. Loss of Tgfbr2 in CNC cells (Wnt1-Cre;Tgfbr2flox/flox) results in microglossia with reduced Scleraxis and Fgf10 expression as well as decreased myogenic cell proliferation, reduced cell number and disorganized tongue muscles. Furthermore, TGF-β2 beads induced the expression of Scleraxis in tongue explant cultures. The addition of FGF10 rescued the muscle cell number in Wnt1-Cre;Tgfbr2flox/flox mice. Thus, TGF-β induced FGF10 signaling has a critical function in regulating tissue-tissue interaction during tongue skeletal muscle development.
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