Mast cells contribute to the resolution of allergic inflammation by releasing resolvin D1.
Mast cells contribute to the resolution of allergic inflammation by releasing resolvin D1.
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肥大细胞通过释放 resolvin D1 有助于解决过敏性炎症。
DOI：
10.1016/j.phrs.2023.106691
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发表时间：
2023
影响因子：
9.3
通讯作者：
Levi-Schaffer,Francesca
中科院分区：
文献类型：
--
作者：
Puzzovio,PierGiorgio;Pahima,Hadas;George,Tresa;Mankuta,David;Eliashar,Ron;Tiligada,Ekaterini;Levy,BruceD;Levi-Schaffer,Francesca
背景肥大细胞与嗜酸性粒细胞一起是过敏性炎症的启动者和主要效应者，它们可以与嗜酸性粒细胞发生物理和可溶性串扰，具有显着的促炎特征，即过敏效应单元。肥大细胞，单独或与嗜酸性粒细胞共培养的前分辨率的作用，尚未characterized yet.ObjectivesWe aimed to investigate selection pro-resolution pathways in mast cellsin vitroandin vivoin allergic inflammation.MethodsIn vitro，我们采用人和小鼠肥大细胞和分析释放的resolvin D1和表达的15-脂氧合酶IgE介导的激活后。我们将IgE激活的肥大细胞与外周血嗜酸性粒细胞共培养，观察15-脂氧合酶的表达和Resolvin D1的释放。aureusenterotoxin B过敏性腹膜炎模型在野生型小鼠后MC“过冲”protocol.ResultsWe发现，IgE激活的肥大细胞释放显着量的消退素D1 30分钟后激活，而15-脂氧合酶的表达保持不变。Resolvin D1的释放被认为是减少IgE激活的肥大细胞与外周血嗜酸性粒细胞共培养30 minIn体内，肥大细胞超负荷小鼠表现出减少炎症的趋势，连同增加腹膜resolvin D1 releasing.ConclusionsMast细胞可以积极地促进决议过敏性炎症释放resolvin D1。
BackgroundMast cells are initiators and main effectors of allergic inflammation, together with eosinophils, with whom they can interact in a physical and soluble cross-talk with marked pro-inflammatory features, the Allergic Effector Unit. The pro-resolution role of mast cells, alone or in co-culture with eosinophils, has not been characterized yet.ObjectivesWe aimed to investigate select pro-resolution pathways in mast cellsin vitroandin vivoin allergic inflammation.MethodsIn vitro, we employed human and murine mast cells and analyzed release of resolvin D1 and expression of 15-lipoxygenase after IgE-mediated activation. We performed co-culture of IgE-activated mast cells with peripheral blood eosinophils and investigated 15-lipoxygenase expression and Resolvin D1 release.In vivo, we performed Ovalbumin/Alum and Ovalbumin/S. aureusenterotoxin B allergic peritonitis model in Wild Type mice following a MC “overshoot” protocol.ResultsWe found that IgE-activated mast cells release significant amounts of resolvin D1 30 min after activation, while 15-lipoxygenase expression remained unchanged. Resolvin D1 release was found to be decreased in IgE-activated mast cells co-cultured with peripheral blood eosinophils for 30 minIn vivo, mast cell-overshoot mice exhibited a trend of reduced inflammation, together with increased peritoneal resolvin D1 release.ConclusionsMast cells can actively contribute to resolution of allergic inflammation by releasing resolvin D1.
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