Induction of inhibitory antibodies to the CCR5 chemokine receptor and their complementary role in preventing SIV infection in macaques
Induction of inhibitory antibodies to the CCR5 chemokine receptor and their complementary role in preventing SIV infection in macaques
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CCR5趋化因子受体抑制性抗体的诱导及其在预防猕猴SIV感染中的互补作用
DOI：
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发表时间：
1999
影响因子：
5.4
通讯作者：
C. Kelly
中科院分区：
文献类型：
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作者：
T. Lehner;Yufei Wang;C. Doyle;L. Tao;L. Bergmeier;E. Mitchell;W. Bogers;J. Heeney;C. Kelly
七个跨膜G蛋白连接的CCR5分子是HIV或猴免疫缺陷病毒(SIV)感染的主要辅助受体。用人CD4+T细胞培养的SIV免疫恒河猴，研究了恒河猴抗CCR5抗体的变化。这些猕猴对静脉注射是完全保护的。用现场SIV挑战。在没有明显的SIV中和抗体的情况下，受保护猕猴的血清对SIV复制(p <； 0.001)和巨噬细胞炎性蛋白-1β产生的CCR5依赖的趋化作用(p <； 0.01)的抑制作用明显强于来自未保护猕猴的血清。这两种功能检测证实了针对CCR5受体的血清抗体，该抗体被CCR5转基因的HEK-293细胞特异性地抑制。我们推测，抗CCR5抗体可能与β趋化因子互补，在阻断CCR5辅助受体与HIV或SIV结合和融合的过程中发挥作用。
The seven‐transmembrane G‐protein‐linked CCR5 molecule functions as a major coreceptor for HIV or simian immunodeficiency virus (SIV) infection. Antibodies to CCR5 were studied in rhesus macaques immunized with SIV grown in human CD4+ T cells. These macaques were completely protected against i.v. challenge with live SIV. Sera from the protected macaques showed significantly greater inhibition of SIV replication (p < 0.001) and macrophage inflammatory protein‐1β‐generated CCR5‐dependent chemotaxis (p < 0.01) than sera from unprotected macaques, in the absence of significant neutralizing antibodies to SIV. These two functional assays demonstrate serum antibodies to the CCR5 receptors which were specifically inhibited by CCR5‐transfected HEK‐293 cells. We postulate that anti‐CCR5 antibodies may be complementary to β‐chemokines in blocking CCR5 coreceptors to HIV or SIV binding and fusion of CD4+ cells.
DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
A. Amara;S. Le Gall;Olivier Schwartz;Jean Salamero;Monica Montes;P. Loetscher;Marco Baggiolini;J. Virelizier;F. Arenzana‐Seisdedos;A. Amara;S. Le Gall
通讯作者：
A. Amara;S. Le Gall;Olivier Schwartz;Jean Salamero;Monica Montes;P. Loetscher;Marco Baggiolini;J. Virelizier;F. Arenzana‐Seisdedos;A. Amara;S. Le Gall
恒河猴免受猿猴免疫缺陷病毒感染的疫苗保护。
影响因子：
1.5
作者：
Carlson,JR;McGraw,TP;Keddie,E;Yee,JL;Rosenthal,A;Langlois,AJ;Dickover,R;Donovan,R;Luciw,PA;Jennings,MB
通讯作者：
Jennings,MB
DOI：
10.1084/jem.184.3.1101
发表时间：
1996-09-01
期刊：
The Journal of experimental medicine
影响因子：
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作者：
Bleul CC;Fuhlbrigge RC;Casasnovas JM;Aiuti A;Springer TA
通讯作者：
Springer TA