Otoprotectant Minimizes Hearing Defects Caused by Pseudomonas aeruginosa Exotoxin A
Otoprotectant Minimizes Hearing Defects Caused by Pseudomonas aeruginosa Exotoxin A
复制标题
耳保护剂可最大限度地减少铜绿假单胞菌外毒素 A 引起的听力缺陷
DOI：
10.1080/000164800750000559
复制
发表时间：
2000
影响因子：
1.4
通讯作者：
R. Popa
中科院分区：
文献类型：
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作者：
R. Popa
由铜绿假单胞菌（PaExoA）产生的外毒素A从中耳渗透到耳蜗中并引起感音神经性听力损失（SNHL）。在这项调查中，我们研究了白化病大鼠的电生理变化后，滴注PaExoA和NG-硝基-L-精氨酸甲酯（L-NAME），一氧化氮合成的抑制剂，到中耳。用听性脑干反应（ABR）技术测定听阈。潜伏期/强度曲线的构建，以区分耳蜗和传导组件的听力损失。PaExoA对耳蜗和SNHL造成损害，主要是在高频下。这种损害被L-NAME阻断。提示一氧化氮可能是细菌毒素所致SNHL机制中的重要环节。L-NAME作为耳保护剂对抗PaExoA的有害作用。
Exotoxin A, produced by Pseudomonas aeruginosa (PaExoA), penetrates from the middle ear in to the cochlea and causes sensorineural hearing loss (SNHL). In this investigation we studied electrophysiological changes in the albino rat following instillation of PaExoA and NG-nitro-L-arginine methyl ester (L-NAME), a known inhibitor of nitric oxide synthesis, into the middle ear. Hearing thresholds were measured by auditory brainstem response (ABR) technique. Latency/intensity curves were constructed to distinguish between cochlear and conductive components of hearing loss. PaExoA caused damage to cochleae and SNHL, mainly at high frequencies. This impairment was blocked by L-NAME. It would appear that nitric oxide may be a significant link in the mechanism of SNHL caused by bacterial toxin. L-NAME acts as an otoprotectant against the deleterious action of PaExoA.
DOI：
10.1073/pnas.89.15.6711
发表时间：
1992-08-01
影响因子：
11.1
作者：
LOWENSTEIN, CJ;GLATT, CS;SNYDER, SH
通讯作者：
SNYDER, SH