Prostate secretions from men with chronic pelvic pain syndrome inhibit proinflammatory mediators.
Prostate secretions from men with chronic pelvic pain syndrome inhibit proinflammatory mediators.
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DOI：
10.1016/j.juro.2010.05.086
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发表时间：
2010-10
期刊：
影响因子：
--
通讯作者：
Schaeffer AJ
中科院分区：
文献类型：
--
作者：
Thumbikat P;Shahrara S;Sobkoviak R;Done J;Pope RM;Schaeffer AJ
在过去，许多趋化因子已被证明是目前在前列腺液（EPS）的CP/CPPS患者。本研究检测了趋化因子在CPPS患者EPS中的功能作用。通过检查单核细胞对MCP-1的趋化性来研究EPS对人单核细胞的功能性影响，MCP-1是先前在CP/CPPS中鉴定的主要化学引诱物。通过定量单核细胞中的细胞内钙升高和良性前列腺上皮细胞中NF-κB的活化来确定对细胞信号传导的影响。我们的研究结果表明，MCP-1存在于EPS是非功能性的，不能介导人类单核细胞的趋化性或介导单核细胞或前列腺上皮细胞中的信号传导。此外，当与来自CPPS患者的EPS一起孵育时，这种功能的缺失可以扩展到其他促炎细胞因子，如IL-1β和TNFα。调节促炎细胞因子的这种明显能力的机制涉及介导免疫和前列腺上皮细胞功能抑制的热不稳定细胞外蛋白酶。这些结果可能对设计针对完全解决前列腺炎症损伤的特定诊断和治疗方法产生影响。
In the past, numerous chemokines have been shown to be present in the expressed prostatic secretions (EPS) of patients with CP/CPPS. This study examined the functional effects of chemokines in the EPS of patients with CPPS. The functional effects of EPS on human monocytes were studied by examining monocyte chemotaxis in response to MCP-1, a major chemoattractant previously identified in CP/CPPS. Effects on cellular signaling were determined by quantifying intracellular calcium elevation in monocytes and activation of NF-κB in benign prostate epithelial cells. Our results show that the MCP-1 present in EPS is non-functional, with an inability to mediate chemotaxis of human monocytes or mediate signaling in either monocytes or prostate epithelial cells. Moreover, this absence of functionality could be extended to other proinflammatory cytokines such as IL-1β and TNFα, when incubated with the EPS from CPPS patients. The mechanism underlying this apparent ability to modulate pro-inflammatory cytokines involves heat labile extracellular proteases that mediate inhibition of both immune and prostate epithelial cell function. These results may have implications for the design of specific diagnostics and therapeutics targeted at complete resolution of prostate inflammatory insults.
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影响因子：
4.4
作者：
Thumbikat, P;Dileepan, T;Maheswaran, SK
通讯作者：
Maheswaran, SK
影响因子：
6.6
作者：
True, LD;Berger, RE;Krieger, JN
通讯作者：
Krieger, JN
影响因子：
7.8
作者：
Foxman, E F;Campbell, J J;Butcher, E C
通讯作者：
Butcher, E C
DOI：
10.1002/pros.21081
发表时间：
2010-04-01
期刊：
The Prostate
影响因子：
--
作者：
Fujita K;Ewing CM;Getzenberg RH;Parsons JK;Isaacs WB;Pavlovich CP
通讯作者：
Pavlovich CP
影响因子：
6.6
作者：
Nickel, J. Curtis;Roehrborn, Claus G.;Rittmaster, Roger S.
通讯作者：
Rittmaster, Roger S.