Genome-wide profiling of microRNAs in adipose mesenchymal stem cell differentiation and mouse models of obesity.
Genome-wide profiling of microRNAs in adipose mesenchymal stem cell differentiation and mouse models of obesity.
复制标题
脂肪间充质干细胞分化和肥胖小鼠模型中microRNA的全基因组分析。
DOI：
10.1371/journal.pone.0021305
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Bielke W
中科院分区：
文献类型：
--
作者：
Bengestrate L;Virtue S;Campbell M;Vidal-Puig A;Hadaschik D;Hahn P;Bielke W
近年来，越来越多的证据表明microrna是基因表达的关键调控分子。受microrna调控的细胞过程包括细胞增殖、细胞程序性死亡和细胞分化。脂肪细胞分化是一个高度调控的细胞过程，已经发现了几个重要的调节因子，但尚未完全了解其潜在机制。在本研究中，我们利用实时RT-PCR分析了小鼠间充质干细胞向终末分化脂肪细胞分化过程中597个microrna的表达。与未分化的祖细胞相比，间充质干细胞衍生的脂肪细胞中总共有66种mirna差异表达。为了进一步研究这66种mirna在体内白色脂肪组织中的调控及其对PPARγ活性的依赖性，我们采用了遗传或饮食诱导的肥胖小鼠模型以及表达显性PPARγ负突变体的小鼠系。
In recent years, there has been accumulating evidence that microRNAs are key regulator molecules of gene expression. The cellular processes that are regulated by microRNAs include e.g. cell proliferation, programmed cell death and cell differentiation. Adipocyte differentiation is a highly regulated cellular process for which several important regulating factors have been discovered, but still not all are known to fully understand the underlying mechanisms. In the present study, we analyzed the expression of 597 microRNAs during the differentiation of mouse mesenchymal stem cells into terminally differentiated adipocytes by real-time RT-PCR. In total, 66 miRNAs were differentially expressed in mesenchymal stem cell-derived adipocytes compared to the undifferentiated progenitor cells. To further study the regulation of these 66 miRNAs in white adipose tissue in vivo and their dependence on PPARγ activity, mouse models of genetically or diet induced obesity as well as a mouse line expressing a dominant negative PPARγ mutant were employed.
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影响因子：
11.2
作者：
Hurst DR;Edmonds MD;Scott GK;Benz CC;Vaidya KS;Welch DR
通讯作者：
Welch DR
影响因子：
46.9
作者：
Canales, Roger D.;Luo, Yuling;Goodsaid, Federico M.
通讯作者：
Goodsaid, Federico M.
影响因子：
6.2
作者：
McPherson, Ruth
通讯作者：
McPherson, Ruth
影响因子：
3.7
作者：
Ortega FJ;Moreno-Navarrete JM;Pardo G;Sabater M;Hummel M;Ferrer A;Rodriguez-Hermosa JI;Ruiz B;Ricart W;Peral B;Fernández-Real JM
通讯作者：
Fernández-Real JM
影响因子：
8.9
作者：
Heneghan, H. M.;Miller, N.;Kerin, M. J.
通讯作者：
Kerin, M. J.