Patchouli alcohol improved diarrhea-predominant irritable bowel syndrome by regulating excitatory neurotransmission in the myenteric plexus of rats.
Patchouli alcohol improved diarrhea-predominant irritable bowel syndrome by regulating excitatory neurotransmission in the myenteric plexus of rats.
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DOI：
10.3389/fphar.2022.943119
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发表时间：
2022
影响因子：
5.6
通讯作者：
Tan, Bo
中科院分区：
文献类型：
--
作者：
Chen, Wanyu;Liao, Lu;Huang, Zitong;Lu, Yulin;Lin, Yukang;Pei, Ying;Yi, Shulin;Huang, Chen;Cao, Hongying;Tan, Bo
关键词：
背景和目的：肠易激综合征(IBS)通常与慢性胃肠道疾病有关。其最常见的亚型是伴有腹泻(IBS-D)。肠神经系统(ENS)调节主要的胃肠动力和功能，其异常可能导致IBS-D。肠道神经元容易受到长期神经递质水平变化的影响。广藿香醇(PA)是从广藿香中提取的一种有效成分，具有调节神经递质释放的作用，但其抗IBS-D的作用及其机制尚不清楚。实验方法：本研究通过慢性束缚应激建立大鼠IBS-D模型。分别给予大鼠5、10和20 mg/kg的PA进行肠道和内脏检查。进一步用免疫组织化学方法对纵肌肌间神经丛(LMMP)神经元进行定量、形态和神经递质分析。主要结果：我们发现PA降低了IBS-D大鼠的内脏敏感性、腹泻症状和肠道传输。同时，10和20 mg/kg的PA显著降低IBS-D大鼠远端结肠兴奋性LMMP神经元的比例，减少远端结肠乙酰胆碱(Ach)和P物质(SP)阳性神经元的数量，并恢复Ach和SP的水平。结论与意义：PA可调节LMMP兴奋性神经元的活动，改善肠动力，减轻IBS引起的腹泻症状，提示PA对IBS-D有潜在的治疗作用。
Background and Purpose: Irritable bowel syndrome (IBS) is usually associated with chronic gastrointestinal disorders. Its most common subtype is accompanied with diarrhea (IBS-D). The enteric nervous system (ENS) modulates major gastrointestinal motility and functions whose aberration may induce IBS-D. The enteric neurons are susceptible to long-term neurotransmitter level alterations. The patchouli alcohol (PA), extracted from Pogostemonis Herba, has been reported to regulate neurotransmitter release in the ENS, while its effectiveness against IBS-D and the underlying mechanism remain unknown. Experimental Approach: In this study, we established an IBS-D model in rats through chronic restraint stress. We administered the rats with 5, 10, and 20 mg/kg of PA for intestinal and visceral examinations. The longitudinal muscle myenteric plexus (LMMP) neurons were further immunohistochemically stained for quantitative, morphological, and neurotransmitters analyses. Key Results: We found that PA decreased visceral sensitivity, diarrhea symptoms and intestinal transit in the IBS-D rats. Meanwhile, 10 and 20 mg/kg of PA significantly reduced the proportion of excitatory LMMP neurons in the distal colon, decreased the number of acetylcholine (Ach)- and substance P (SP)-positive neurons in the distal colon and restored the levels of Ach and SP in the IBS-D rats. Conclusion and Implications: These findings indicated that PA modulated LMMP excitatory neuron activities, improved intestinal motility and alleviated IBS-induced diarrheal symptoms, suggesting the potential therapeutic efficacy of PA against IBS-D.
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