Preliminary screening and analysis of metastasis-related lncRNA and co-expressed papillary thyroid carcinoma mRNA.
Preliminary screening and analysis of metastasis-related lncRNA and co-expressed papillary thyroid carcinoma mRNA.
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转移相关lncRNA及共表达甲状腺乳头状癌mRNA的初步筛选与分析
DOI：
10.3892/ol.2018.9080
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发表时间：
2018-09
期刊：
影响因子：
2.9
通讯作者：
Cai XJ
中科院分区：
文献类型：
--
作者：
Ge MH;Jiang LH;Wen QL;Tan Z;Chen C;Zheng CM;Zhu X;Chen JW;Zhu ZY;Cai XJ
本研究的目的是探讨与甲状腺乳头状癌（PTC）侵袭和转移相关的长非编码RNA（lncRNA）和mRNA表达谱。 Transwell侵袭测定用于筛选人PTC IHH4细胞系的三种高侵袭性亚株：IHH4-M1、IHH4-M2和IHH4-M3。此外，还利用荷瘤裸鼠来鉴定这三个亚株的侵袭和转移能力。采用安捷伦lncRNA微阵列芯片筛选了795个差异表达的lncRNA和788个差异表达的mRNA。随机选取10个lncRNA和10个mRNA进行RT-qPCR验证，确认结果与微阵列芯片一致，表明微阵列芯片分析结果相对准确。基于基因本体富集的聚类分析显示，差异表达基因主要与类固醇生物合成、生物粘附、细胞间粘附和其他转移相关的生物过程有关。通路聚类分析结果发现差异表达基因与肿瘤转移相关信号通路相关，包括胆固醇代谢信号通路、甾醇调节元件结合蛋白信号通路和整合素信号通路，提示lncRNA可能通过多种信号通路调节PTC转移。本研究筛选并构建了PTC转移相关的lncRNA和mRNA表达谱，为今后PTC高危分子标志物的研究提供了分子基础。
The objective of the present study was to investigate the long non-coding RNA (lncRNA) and mRNA expression profiles that are associated with the invasion and metastasis of papillary thyroid carcinoma (PTC). Transwell invasion assays were used to screen three highly invasive sub-strains of the human PTC IHH4 cell line: IHH4-M1, IHH4-M2 and IHH4-M3. In addition, tumor-bearing nude mice were used to identify the invasive and metastatic capacity of the three sub-strains. Agilent lncRNA microarray chips were used to screen 795 differentially expressed lncRNAs and 788 differentially expressed mRNAs. A total of 10 lncRNAs and 10 mRNAs were randomly selected for RT-qPCR validation to confirm that the results were consistent with the microarray chips, suggesting that the results of the microarray chip analysis were relatively accurate. Gene ontology enrichment-based cluster analysis revealed that the differentially expressed genes were mainly associated with steroid biosynthesis, bioadhesion, intercellular adhesion and other metastasis-associated biological processes. The results of the pathway cluster analysis identified that the differentially expressed genes were associated with tumor metastasis-associated signaling pathways, including the cholesterol metabolic signaling pathway, the sterol regulatory element-binding protein signaling pathway and the integrin signaling pathway, suggesting that lncRNA may regulate PTC metastasis through various signaling pathways. The present study screened and constructed PTC metastasis-associated lncRNA and mRNA expression profiles, and it provides a molecular basis for the future study of high-risk molecular markers of PTC.
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影响因子：
14.9
作者：
Moran VA;Perera RJ;Khalil AM
通讯作者：
Khalil AM
影响因子：
8
作者：
Braconi, C.;Kogure, T.;Valeri, N.;Huang, N.;Nuovo, G.;Costinean, S.;Negrini, M.;Miotto, E.;Croce, C. M.;Patel, T.
通讯作者：
Patel, T.
影响因子：
5.8
作者：
Durante, C.;Haddy, N.;Schlumberger, M.
通讯作者：
Schlumberger, M.
影响因子：
--
作者：
Liu, Jiang-Hua;Chen, Gang;Luo, Dian-Zhong
通讯作者：
Luo, Dian-Zhong
影响因子：
6.4
作者：
Joung, Ji Y.;Kim, Tae H.;Kim, Sun W.
通讯作者：
Kim, Sun W.