The Early Diagnostic Value of Serum Interleukin-8 in Esophagogastric Junction Adenocarcinoma.
The Early Diagnostic Value of Serum Interleukin-8 in Esophagogastric Junction Adenocarcinoma.
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DOI：
10.1177/10732748211004883
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Li Z;Xu H;Yu J;Liu C;Zheng C;Zeng R;Xu L;Li E;Peng Y;Xu Y
食管胃结合部腺癌（EJA）是消化道最常见的恶性肿瘤之一，病死率高。由于缺乏早期诊断生物标志物，EJA的预后很差。目前迫切需要早期EJA的非侵入性生物标志物。目的探讨血清白细胞介素-8（IL-8）水平对EJA的早期诊断价值。基于癌症基因组图谱（TCGA）数据库的贲门腺癌样品分析IL-8 mRNA表达数据。采用酶联免疫吸附法（ELISA）检测了2所肿瘤医院95例EJA患者和95例正常对照者血清IL-8水平。采用Mann-Whitney U检验和受试者工作特征（ROC）曲线评价血清IL-8水平的诊断准确性。EJA组IL-8 mRNA表达水平和血清IL-8水平均显著高于正常组（P均< 0.001）。ROC曲线下面积（AUC）为0.661（95% CI，0.583-0.740）和0.745（95%CI，0.606-0.885），敏感性为43.2%以109.086 pg/mL为最佳临界值，EJA组和早期EJA组的特异性分别为87.4%（95%CI，78.6%~ 93.1%）和66.7%（95%CI，33.2%~ 53.7%）。临床资料分析显示，患者性别、浸润深度、淋巴结转移、TNM分期与血清IL-8水平有显著相关性（均P < 0.05）。血清IL-8是一个潜在的诊断生物标志物，以确定早期EJA。
Esophagogastric junction adenocarcinoma (EJA) is one of the most common malignant tumors of digestive tract with high mortality worldwide. Given a lack of early diagnosis biomarkers, the prognosis of EJA is poor. Non-invasive biomarkers for early-stage EJA are urgently required. We aimed at evaluating the early diagnostic value of serum interleukin-8 (IL-8) level in EJA patients. The IL-8 mRNA expression data were analyzed based on the stomach cardia adenocarcinoma samples of The Cancer Genome Atlas (TCGA) database. Enzyme-linked immunosorbent assay (ELISA) was used to measure the concentration of serum IL-8 in 95 EJA patients and 95 normal controls enrolled from 2 different cancer hospitals. The diagnostic accuracy of serum IL-8 was evaluated by applying Mann-Whitney U test and receiver operating characteristic (ROC) curve. The mRNA expression levels and serum levels of IL-8 in EJA group were significantly higher than those in the normal group (all P < 0.001). The areas under the ROC curve (AUC) were 0.661 (95% CI, 0.583-0.740) and 0.745 (95% CI, 0.606-0.885), with the sensitivities of 43.2% (95% CI, 33.2%-53.7%) and 66.7% (95% CI, 46.0%-82.8%) and the specificities of 87.4% (95% CI, 78.6%-93.1%) in EJA group and early-EJA group, respectively, when the optimal cutoff value was 109.086 pg/mL. The clinical data analysis showed there were significant correlations between patient genders, depth of invasion, lymph node metastasis, TNM stage and the serum level of IL-8 (all P < 0.05). Serum IL-8 represents a potential diagnostic biomarker to identify early-stage EJA.
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影响因子：
12.4
作者：
Ha H;Debnath B;Neamati N
通讯作者：
Neamati N
DOI：
10.1002/hep.29561
发表时间：
2018-03
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Luo P;Yin P;Hua R;Tan Y;Li Z;Qiu G;Yin Z;Xie X;Wang X;Chen W;Zhou L;Wang X;Li Y;Chen H;Gao L;Lu X;Wu T;Wang H;Niu J;Xu G
通讯作者：
Xu G
DOI：
10.2174/1871520615666150716105255
发表时间：
2015-01-01
影响因子：
2.8
作者：
Poletaev, Alexander;Pukhalenko, Alexander;Sviridov, Pavel
通讯作者：
Sviridov, Pavel
影响因子：
9.8
作者：
Isomoto, H;Saenko, VA;Kohno, S
通讯作者：
Kohno, S
影响因子：
10.3
作者：
Pine, Sharon R.;Mechanic, Leah E.;Harris, Curtis C.
通讯作者：
Harris, Curtis C.