Oxidative Damage and Nrf2 Translocation Induced by Toxicities of Deoxynivalenol on the Placental and Embryo on Gestation Day 12.5 d and 18.5 d.
Oxidative Damage and Nrf2 Translocation Induced by Toxicities of Deoxynivalenol on the Placental and Embryo on Gestation Day 12.5 d and 18.5 d.
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脱氧雪腐镰刀菌烯醇毒性对妊娠12.5天和18.5天胎盘和胚胎的氧化损伤和Nrf2易位
DOI：
10.3390/toxins10090370
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发表时间：
2018-09-13
期刊：
影响因子：
4.2
通讯作者：
Chen JH
中科院分区：
文献类型：
--
作者：
Yu M;Wei ZY;Xu ZH;Pan JQ;Chen JH
脱氧雪腐镰刀菌烯醇（DON）是一种属于单端孢霉烯族的天然污染物。本研究的目的是使用多种检测方法来评估氧化损伤以及核因子红细胞 2 相关因子 2 (Nrf2) 的易位，并研究其在 DON 诱导的胎盘和胚胎毒性中的机制。怀孕的C57BL/6小鼠被随机分为三组，分别接受不同剂量的DON：0、1.0、2.5 mg/(kg·天)。在妊娠日 (GD) 12.5 天和 18.5 天，DON 导致胚胎吸收率升高以及胎盘结构和功能损伤。在胎盘中，抗氧化酶丙二醛、超氧化物歧化酶和谷胱甘肽水平的改变表明存在显着的氧化应激。此外，DON-L组（1.0 mg/（kg·day））血红素加氧酶-1（HO-1）水平升高和Nrf2从细胞核易位到细胞质表明Nrf2/HO-1途径激活。值得注意的是，本实验在 GD 12.5 d 的结果与 GD 18.5 d 的结果相似。总之，DON 诱导的胎盘氧化损伤和 Nrf2 易位在 GD 12.5 d 和 GD 18.5 d 时相似。氧化应激是 DON 诱导的胚胎毒性最重要的分子机制之一，Nrf2 易位可能在对抗氧化应激方面发挥重要作用。
Deoxynivalenol (DON) is a kind of natural pollutant belonging to the trichothecenes family. The aim of this study is to use diverse assays to evaluate oxidative damage as well as translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), and to investigate their mechanisms in DON-induced toxicities on a placenta and embryo. Pregnant C57BL/6 mice were randomly assigned to three groups with different doses of DON: 0, 1.0, 2.5 mg/(kg·day). In gestation day (GD) 12.5 d and 18.5 d, DON induced an elevated resorption rate of the embryos as well as structural and functional damage of the placenta. In the placenta, altered levels of the antioxidant enzymes malondialdehyde, superoxide dismutase and glutathione indicated remarkable oxidative stress. Furthermore, an elevated level of heme oxygenase-1 (HO-1) and the translocation of Nrf2 from nucleus to cytoplasm indicated Nrf2/HO-1 pathway activation in DON-L group (1.0 mg/(kg·day)). It is noteworthy that the results in this experiment in GD 12.5 d were similar to those in GD 18.5 d. In conclusion, DON-induced placental oxidative damage and Nrf2 translocation were similar in GD 12.5 d and GD 18.5 d. Oxidative stress is one of the most important molecular mechanisms for embryotoxicity induced by DON, and Nrf2 translocation may play a substantial role against it.
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影响因子：
4.7
作者：
Mitsuishi Y;Motohashi H;Yamamoto M
通讯作者：
Yamamoto M
影响因子：
3.3
作者：
Katika, Madhumohan R.;Hendriksen, Peter J. M.;Peijnenburg, Ad A. C. M.
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Peijnenburg, Ad A. C. M.
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Motterlini, R
影响因子：
3.8
作者：
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通讯作者：
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