Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP.
Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP.
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DOI：
10.1038/s41598-017-14466-x
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发表时间：
2017-11-02
影响因子：
4.6
通讯作者：
Nishimaki-Mogami T
中科院分区：
文献类型：
--
作者：
Kondo K;Nakamura K;Ishigaki T;Sakata K;Obitsu S;Noguchi A;Fukuda N;Nagasawa E;Teshima R;Nishimaki-Mogami T
有毒的红缘内孔菌Entoloma rhodopolium和其他类似物种，包括食用内孔菌E.疥螨在形态上是多样的。人们把有毒的物种误认为是可食用的物种。需要确定这些物种的分类和检测方法。在北方欧洲已有形态学和系统发育学的研究报道。在日本，基因研究仍然没有解决。因此，E.利用ITS和RPB2序列对红藻属的12个种进行了分析，并与欧洲种的结果进行了比较。日本E. rhodopolium分为3个分支，均不属于真正的欧洲E. Rhodopolium和其他已知的物种。其中3个种为新种。红柱内瘤菌（Entoloma rhodopolium clade-I）命名为E. lacus）与E. majaloides。Clade-II（E. subrhodopolium）与E. sinuatum和E. subsinuatum，但不同于这些种。进化枝-III从已知的Entoloma物种包括E. lupinum，命名为E.假紫质土在此基础上，建立了一种简单的鉴别方法PCR-RFLP，用于区分有毒种和食用种。sarcopum，这是非常相似的形态。该研究有助于阐明复合体E.红藻相关物种，并防止食物中毒。
Poisonous Entoloma rhodopolium and other similar species including edible E. sarcopum are morphologically diverse. People mistake poisonous species for edible species. Classification and the detection method of these species need to be defined. The morphological and phylogenetic studies have been reported in northern Europe. In Japan, the genetic study remains unsolved. Thus, phylogenetic analysis of E. rhodopolium was conducted using ITS and RPB2 sequences, and the result was compared with that of European species. Japanese E. rhodopolium was classified into three clades, none of which belonged to the true European E. rhodopolium and other known species. Three species were defined as new species. Entoloma rhodopolium clade-I (named E. lacus) was genetically close to but morphologically separated from E. majaloides. Clade-II (E. subrhodopolium) was classified to the same group as E. sinuatum and E. subsinuatum, but distinct from these species. Clade-III was segregated from known Entoloma species including E. lupinum, and named E. pseudorhodopolium. Based on the classification, a simple identification method PCR-RFLP was developed to discriminate between poisonous species and edible E. sarcopum, which is very similar in morphology. The study can help to clarify the taxonomy of complex E. rhodopolium-related species, and to prevent food poisoning.
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